ArticleAnnals of biomedical engineering2020
Dynamics of Cell Death After Conventional IRE and H-FIRE Treatments.
Article in Annals of biomedical engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 2 syntheses or guidelines pooled it, 83 citations in OpenAlex.
- Electrochemotherapy in pediatrics: a systematic review.European radiology · 2026Pooled it
- Pulsed field ablation for the interventional treatment of atrial fibrillation: a scientific statement of the European Heart Rhythm Association of the European Society of Cardiology, the Heart Rhythm Society, the Asia Pacific Heart Rhythm Society, the Latin American Heart Rhythm Society, and the Canadian Heart Rhythm Society.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2026Guideline
- Parameter-Dependent Electroporation and Cellular Responses Induced by Microsecond Pulsed Electric Fields: Different Sensitivity of Two Pancreatic Ductal Adenocarcinoma Cell Lines.Bioengineering (Basel, Switzerland) · 2026Article
- [Coupling characteristics of cellular electroporation and electrodeformation based on dynamic Young's modulus: influence of membrane thickness].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2026Article
- Biomaterial-Integrated Electroporation for Therapeutic Delivery: From Gene Editing to Tumor Ablation and Immune Modulation.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- High-frequency irreversible electroporation versus transurethral resection of the prostate for benign prostatic hyperplasia (GIANT): a single-centre, randomised, double-blind, phase 3, non-inferiority trial.EClinicalMedicine · 2026Article
- AAV9-Mimetic Peptides and Electroporation Synergistically Enhance Nanoparticle Transport through the Blood-Brain Barrier.ACS biomaterials science & engineering · 2026Article
- Disruption of iron homeostasis sensitizes pancreatic cancer to irreversible electroporation.Nature communications · 2026Article
- Novel Combination of Irreversible Electroporation and Allogenic Chimeric Antigen Receptor T-Cell Therapy Synergizes Therapeutic Outcomes in a Preclinical Human Pancreatic Cancer Mouse Model.Research (Washington, D.C.) · 2026Article
- Mitigating muscle contractions during pulsed field ablation by utilizing intersegment delays.Bioelectrochemistry (Amsterdam, Netherlands) · 2025Article
- Electroporation in lipid vesicles by varying PEG-grafted lipids in their membranes: an experimental and simulation study.RSC advances · 2025Article
- In Situ Vaccination by Tumor Ablation: Principles and Prospects for Systemic Antitumor Immunity.Vaccines · 2025Review
- Effects of Interphase and Interpulse Delays on Tissue Impedance and Pulsed Field Ablation.Annals of biomedical engineering · 2025Article
- Pulsed field ablation in medicine: irreversible electroporation and electropermeabilization theory and applications.Radiology and oncology · 2025Review
- Toward Large Ablations With Single-Needle High-Frequency Irreversible Electroporation In Vivo.IEEE transactions on bio-medical engineering · 2025Article
- Complexity and Perplexity of Pulsed Field Ablation: An Engineering Perspective.Arrhythmia & electrophysiology review · 2025Review
- Biophysics and electrophysiology of pulsed field ablation in normal and infarcted porcine cardiac ventricular tissue.Scientific reports · 2024Article
- Modulating the Cell Death Immune Response for Electroporation Treatments.Bioelectricity · 2024Article
- Advances in irreversible electroporation for prostate cancer.Discover oncology · 2024Review
- Irreversible Electroporation Margin Accentuation in Pancreaticoduodenectomy: A Propensity Score Matching Analysis.Annals of surgical oncology · 2024Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
High-frequency irreversible electroporation (H-FIRE) has emerged as an alternative to conventional irreversible electroporation (IRE) to overcome the issues associated with neuromuscular electrical stimulation that appear in IRE treatments. In H-FIRE, the monopolar pulses typically used in IRE are replaced with bursts of short bipolar pulses. Currently, very little is known regarding how the use of a different waveform affects the cell death dynamics and mechanisms. In this study, human pancreatic adenocarcinoma cells were treated with a typical IRE protocol and various H-FIRE schemes with the same energized time. Cell viability, membrane integrity and Caspase 3/7 activity were assessed at different times after the treatment. In both treatments, we identified two different death dynamics (immediate and delayed) and we quantified the electric field ranges that lead to each of them. While in the typical IRE protocol, the electric field range leading to a delayed cell death is very narrow, this range is wider in H-FIRE and can be increased by reducing the pulse length. Membrane integrity in cells suffering a delayed cell death shows a similar time evolution in all treatments, however, Caspase 3/7 expression was only observed in cells treated with H-FIRE.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.