ArticleHorticulture research2020
A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress.
Article in Horticulture research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Re-analysis of mobile mRNA datasets raises questions about the extent of long-distance mRNA communication.Nature plants · 2025Pooled it
- Integrated multi-omics analysis reveals the molecular mechanism underlying poplar 107 rootstock-mediated regulation ofHorticulture research · 2026Article
- Genome-Wide Survey and Expression Profiling of bZIP Transcription Factors inInternational journal of molecular sciences · 2026Article
- Rootstock-Scion Exchanging mRNAs Participate in Watermelon Fruit Quality Improvement.International journal of molecular sciences · 2025Article
- Identification of Long-Distance Mobile mRNAs Responding to Drought Stress in Heterografted Tomato Plants.International journal of molecular sciences · 2025Article
- Mobile RNAs as systemic signaling beyond boundaries for plant stress resistance.Frontiers in plant science · 2025Review
- m6A and m5C modifications as the gears: CmoCK1 mRNA travels to promote chilling tolerance.Plant physiology · 2024Article
- Near-gapless and haplotype-resolved apple genomes provide insights into the genetic basis of rootstock-induced dwarfing.Nature genetics · 2024Article
- Identification of genes associated with the regulation of cold tolerance and the RNA movement in the grafted apple.Scientific reports · 2023Article
- The mRNA mobileome: challenges and opportunities for deciphering signals from the noise.The Plant cell · 2023Review
- Scion-to-Rootstock Mobile Transcription FactorInternational journal of molecular sciences · 2022Article
- Exact Bayesian inference for the detection of graft-mobile transcripts from sequencing data.Journal of the Royal Society, Interface · 2022Article
- Recent Advances in the Analysis of Cold Tolerance in Maize.Frontiers in plant science · 2022Review
- Insight into the bZIP gene family inFrontiers in plant science · 2022Article
- Genome-wide identification and analysis of GRAS transcription factors in the bottle gourd genome.Scientific reports · 2020Article
- Identification of Long-Distance Transmissible mRNA between Scion and Rootstock in Cucurbit Seedling Heterografts.International journal of molecular sciences · 2020Article
- Long-Distance Movement of mRNAs in Plants.Plants (Basel, Switzerland) · 2020Review
- Correction to: A universal pipeline for mobile mRNA detection and insights into heterografting advantages under chilling stress.Horticulture research · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterografting has long been used to enhance the chilling tolerance of temperature-sensitive crops, including watermelon, whose mechanism is known to involve bidirectional long-distance mRNA movements. Despite several studies reporting on mobile mRNA (mb-mRNA) profiles in plants, accurate identification of mb-mRNAs is challenging owing to an array of technical problems. Here, we developed a bioinformatical pipeline that took most of the known technical concerns into consideration and is considered to be a universal tool for mb-mRNA detection in heterografts. By applying this pipeline to a commercial watermelon-bottle gourd heterografting system, we detected 130 and 1144 mb-mRNAs upwardly and 167 and 1051 mb-mRNAs downwardly transmitted under normal and chilling-stress conditions, respectively. Quantitative real-time PCR indicated a high accuracy rate (88.2%) of mb-mRNA prediction with our pipeline. We further revealed that the mobility of mRNAs was not associated with their abundance. Functional annotation and classification implied that scions may convey the stress signal to the rootstock, subsequently triggering energy metabolism reprogramming and abscisic acid-mediated stress responses by upward movement of effective mRNAs, ultimately leading to enhanced chilling tolerance. This study provides a universal tool for mb-mRNA detection in plant heterografting systems and novel insights into heterografting advantages under chilling stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.