ArticleLight, science & applications2020
Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome.
Article in Light, science & applications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 132 citations in OpenAlex.
- Advances in label-free dynamic imaging of live cells using fourier ptychographic microscopy.Biophysics reports · 2026Article
- Optical diffraction tomographic microscopy: a cutting-edge label-free three-dimensional bioimaging.Biophysics reports · 2026Article
- Chem-SIM: SIM-resolved chemical imaging via wide-field mid-infrared photothermal modulation of fluorescence.Nature communications · 2026Article
- Adipocyte-Derived Leptolin Enhances Energy Expenditure and Prevents Obesity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Virtual Overlay Staining With Plasmonic Oligomer Metasurface.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Multimodal holotomography for volumetric monitoring of host-pathogen interactions.Biomedical engineering online · 2026Article
- Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation.iScience · 2026Article
- Intelligent Stain-Free Histology on Structural Colorimetric Nanocavities.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The mechanisms and functions of mitochondria-encapsulating extracellular vesicles.Science China. Life sciences · 2026Review
- ER tethering and active transport govern condensate diffusion during hyperosmotic stress.Genome biology · 2026Article
- Label-free imaging of intracellular structures in living mammalian cells via external apodization phase-contrast microscopy.The FEBS journal · 2026Article
- Point spread function decoupling in computational fluorescence microscopy.Light, science & applications · 2026Article
- A guide to characterizing the dynamic mitochondria-endoplasmic reticulum contact sites.The FEBS journal · 2025Review
- High-throughput marker-free screening of cytoplasmic vacuoles in adherent cells via Fourier ptychographic microscopy.Biomedical optics express · 2025Article
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- Fourier synthesis optical diffraction tomography for kilohertz rate volumetric imaging.Science advances · 2025Article
- Single particle fluorescence imaging of perovskite nanocrystal crystallization for illustrating coupled nucleation-and-growth.Nature communications · 2025Article
- Pulsed electromagnetic fields modulate energy metabolism during wound healing process: an in vitro model study.BMC complementary medicine and therapies · 2025Article
- Multi-modal transport of intensity diffraction tomography microscopy with an electrically tunable lens [Invited].Biomedical optics express · 2025Article
- The inter-organelle cross-talk finely orchestrated in the amyloidogenic processing of amyloid precursor protein in dendritic arborization neurons ofTheranostics · 2025Article
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of super-resolution (SR) fluorescence microscopy has rejuvenated the search for new cellular sub-structures. However, SR fluorescence microscopy achieves high contrast at the expense of a holistic view of the interacting partners and surrounding environment. Thus, we developed SR fluorescence-assisted diffraction computational tomography (SR-FACT), which combines label-free three-dimensional optical diffraction tomography (ODT) with two-dimensional fluorescence Hessian structured illumination microscopy. The ODT module is capable of resolving the mitochondria, lipid droplets, the nuclear membrane, chromosomes, the tubular endoplasmic reticulum, and lysosomes. Using dual-mode correlated live-cell imaging for a prolonged period of time, we observed novel subcellular structures named dark-vacuole bodies, the majority of which originate from densely populated perinuclear regions, and intensively interact with organelles such as the mitochondria and the nuclear membrane before ultimately collapsing into the plasma membrane. This work demonstrates the unique capabilities of SR-FACT, which suggests its wide applicability in cell biology in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.