ArticlePlant methods2020
A cucumber green mottle mosaic virus vector for virus-induced gene silencing in cucurbit plants.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 89 citations in OpenAlex.
- RNA interference mediated disease resistance in vegetable crops: fundamental discovery to current trends.Molecular biology reports · 2026Review
- Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.Genetics and molecular biology · 2026Article
- APathogens (Basel, Switzerland) · 2025Article
- Comparative transcriptome analysis reveals the role of CsABA8ox1 in cucumber under high-temperature stress.Plant molecular biology · 2025Article
- Cucumber Green Mottle Mosaic Virus Decreases ChlorophyllPlants (Basel, Switzerland) · 2025Article
- HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon.Molecular plant pathology · 2025Article
- Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants.Stress biology · 2025Article
- Multi-omics analyses unveil dual genetic loci governing four distinct watermelon flesh color phenotypes.Molecular horticulture · 2025Article
- Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.Molecular plant pathology · 2025Article
- Comparative transcriptomics of susceptible and resistant Cucumis metuliferus upon Meloidogyne incognita infection.Planta · 2025Article
- Article
- Identification of Candidate Genes for Green Rind Color in Watermelon.Plants (Basel, Switzerland) · 2025Article
- A Bifunctional Nuclease Promotes the Infection of Zucchini Yellow Mosaic Virus in Watermelon by Targeting P3.Plants (Basel, Switzerland) · 2024Article
- Genetic dissection of Meloidogyne incognita resistance genes based on VIGS functional analysis in Cucumis metuliferus.BMC plant biology · 2024Article
- A genome-wide association study reveals molecular mechanism underlying powdery mildew resistance in cucumber.Genome biology · 2024Article
- Fine mapping and identification of ERF transcription factor ERF017 as a candidate gene for cold tolerance in pumpkin.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- A multifaceted crosstalk between brassinosteroid and gibberellin regulates the resistance of cucumber to Phytophthora melonis.The Plant journal : for cell and molecular biology · 2024Article
- Transcriptome Analysis Reveals the Molecular Mechanism of the Leaf Yellowing in Allotriploid Cucumber.Genes · 2024Article
- A large presence/absence variation in the promotor of the ClLOG gene determines trichome elongation in watermelon.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- RNA-seq-based comparative transcriptome analysis reveals the role ofHorticulture research · 2024Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCucurbits produce fruits or vegetables that have great dietary importance and economic significance worldwide. The published genomes of at least 11 cucurbit species are boosting gene mining and novel breeding strategies, however genetic transformation in cucurbits is impractical as a tool for gene function validation due to low transformation efficiency. Virus-induced gene silencing (VIGS) is a potential alternative tool. So far, very few ideal VIGS vectors are available for cucurbits.
resultsHere, we describe a new VIGS vector derived from cucumber green mottle mosaic virus (CGMMV), a monopartite virus that infects cucurbits naturally. We show that the CGMMV vector is competent to induce efficient silencing of the phytoene desaturase (
conclusionsThese results demonstrate that CGMMV vector could serve as a powerful and easy-to-use tool for characterizing gene function, controlling viral pathogens or even performing resistance breeding in cucurbits. Moreover, this study will possess considerable important reference value for developing different viral vectors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.