ArticleNature communications2020
Pre-processing visualization of hyperspectral fluorescent data with Spectrally Encoded Enhanced Representations.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Deep-Learning-Driven High-Fidelity In Vivo Hyperspectral Fluorescence Imaging Under Extreme Photon-Limited Conditions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Transforming hyperspectral data into insight: the DREAM approach for pathology.Biomedical optics express · 2025Article
- Hyperspectral Fluorescence Imaging with a New Polarity-Ultrasensitive Fluorescent Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Noninvasive Oral Hyperspectral Imaging-Driven Digital Diagnosis of Heart Failure With Preserved Ejection Fraction: Model Development and Validation Study.Journal of medical Internet research · 2025Article
- A spontaneous keypoints connection algorithm for leafy plants skeletonization and phenotypes extraction.Frontiers in plant science · 2025Article
- Article
- Multidimensional quantitative characterization of basal cell carcinoma by spectral- and time-resolved two-photon microscopy.Nanophotonics (Berlin, Germany) · 2024Article
- Information-Rich Multi-Functional OCT for Adult Zebrafish Intra- and Extracranial Imaging.Bioengineering (Basel, Switzerland) · 2023Article
- A single-shot hyperspectral phasor camera for fast, multi-color fluorescence microscopy.Cell reports methods · 2023Article
- Neural Networks for Hyperspectral Imaging of Historical Paintings: A Practical Review.Sensors (Basel, Switzerland) · 2023Review
- SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole.Scientific reports · 2023Article
- HyU: Hybrid Unmixing for longitudinal in vivo imaging of low signal-to-noise fluorescence.Nature methods · 2023Article
- A phasor-based approach to improve optical sectioning in any confocal microscope with a tunable pinhole.Microscopy research and technique · 2022Article
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis.Nature communications · 2022Article
- Rapid statistical discrimination of fluorescence images of T cell receptors on immobilizing surfaces with different coating conditions.Scientific reports · 2021Article
- Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications.Communications biology · 2021Article
- Phasor-based image segmentation: machine learning clustering techniques.Biomedical optics express · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Hyperspectral fluorescence imaging is gaining popularity for it enables multiplexing of spatio-temporal dynamics across scales for molecules, cells and tissues with multiple fluorescent labels. This is made possible by adding the dimension of wavelength to the dataset. The resulting datasets are high in information density and often require lengthy analyses to separate the overlapping fluorescent spectra. Understanding and visualizing these large multi-dimensional datasets during acquisition and pre-processing can be challenging. Here we present Spectrally Encoded Enhanced Representations (SEER), an approach for improved and computationally efficient simultaneous color visualization of multiple spectral components of hyperspectral fluorescence images. Exploiting the mathematical properties of the phasor method, we transform the wavelength space into information-rich color maps for RGB display visualization. We present multiple biological fluorescent samples and highlight SEER's enhancement of specific and subtle spectral differences, providing a fast, intuitive and mathematical way to interpret hyperspectral images during collection, pre-processing and analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.