Evidence map›Paper›PMID 32024662›Full record

ArticleGenome research2020

Direct full-length RNA sequencing reveals unexpected transcriptome complexity during

Runsheng Li, Xiaoliang Ren, Qiutao Ding, Yu Bi, Dongying Xie, Zhongying Zhao

Open access · bronzeAbstract read
In one paragraph

Article in Genome research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 70 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Runsheng Li *Department of Biology, Hong Kong Baptist University, Hong Kong, 999077, China.
Xiaoliang Ren *Department of Biology, Hong Kong Baptist University, Hong Kong, 999077, China.
Qiutao DingDepartment of Biology, Hong Kong Baptist University, Hong Kong, 999077, China.
Yu BiDepartment of Biology, Hong Kong Baptist University, Hong Kong, 999077, China.
Dongying XieDepartment of Biology, Hong Kong Baptist University, Hong Kong, 999077, China.
Zhongying ZhaoDepartment of Biology, Hong Kong Baptist University, Hong Kong, 999077, China.ORCID 0000-0003-2743-9008
Hong Kong Baptist University · HK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Massively parallel sequencing of the polyadenylated RNAs has played a key role in delineating transcriptome complexity, including alternative use of an exon, promoter, 5' or 3' splice site or polyadenylation site, and RNA modification. However, reads derived from the current RNA-seq technologies are usually short and deprived of information on modification, compromising their potential in defining transcriptome complexity. Here, we applied a direct RNA sequencing method with ultralong reads using Oxford Nanopore Technologies to study the transcriptome complexity in

Indexed as

AnimalsCaenorhabditis elegansExonsGene Expression ProfilingGene Expression Regulation, DevelopmentalRNARNA, MessengerSequence Analysis, RNATranscriptomeRNARNA, Messenger

Identifiers

PMID32024662
PMCPMC7050527
OpenAlexW3004933912

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.