ArticleCells2020
An Automated, Single Cell Quantitative Imaging Microscopy Approach to Assess Micronucleus Formation, Genotoxicity and Chromosome Instability.
Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- An efficient cell micronucleus classification network based on multi-layer perception attention mechanism.Scientific reports · 2025Article
- micronuclAI enables automated quantification of micronuclei for assessment of chromosomal instability.Communications biology · 2025Article
- Loss of EMI1 compromises chromosome stability and is associated with cellular transformation in colonic epithelial cell contexts.British journal of cancer · 2024Article
- Application of image-recognition techniques to automated micronucleus detection in the in vitro micronucleus assay.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2024Article
- Evaluating Chromosome Instability and Genotoxicity Through Single Cell Quantitative Imaging Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Digital World of Cytogenetic and Cytogenomic Web Resources.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The progress in our understanding of CIN in breast cancer research.Frontiers in oncology · 2023Review
- ReducedCells · 2022Article
- CRISPRthripsis: The Risk of CRISPR/Cas9-induced Chromothripsis in Gene Therapy.Stem cells translational medicine · 2022Article
- The F-box protein, FBXO7, is required to maintain chromosome stability in humans.Human molecular genetics · 2022Article
- Plant Cytogenetics in the Micronuclei Investigation-The Past, Current Status, and Perspectives.International journal of molecular sciences · 2022Review
- Article
- ReducedCancers · 2021Article
- Investigation of cytotoxicity and genotoxicity of abamectin pesticide in Allium cepa L.Environmental science and pollution research international · 2021Article
- ER Stress and Micronuclei Cluster: Stress Response Contributes to Genome Chaos in Cancer.Frontiers in cell and developmental biology · 2021Article
- Genomic Instability in Circulating Tumor Cells.Cancers · 2020Review
- Chromosome Instability; Implications in Cancer Development, Progression, and Clinical Outcomes.Cancers · 2020Review
- ReducedCancers · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Micronuclei are small, extranuclear bodies that are distinct from the primary cell nucleus. Micronucleus formation is an aberrant event that suggests a history of genotoxic stress or chromosome mis-segregation events. Accordingly, assays evaluating micronucleus formation serve as useful tools within the fields of toxicology and oncology. Here, we describe a novel micronucleus formation assay that utilizes a high-throughput imaging platform and automated image analysis software for accurate detection and rapid quantification of micronuclei at the single cell level. We show that our image analysis parameters are capable of identifying dose-dependent increases in micronucleus formation within three distinct cell lines following treatment with two established genotoxic agents, etoposide or bleomycin. We further show that this assay detects micronuclei induced through silencing of the established chromosome instability gene,
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.