ArticleStructure (London, England : 1993)2020
Experimental Phasing of MicroED Data Using Radiation Damage.
Article in Structure (London, England : 1993), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Membrane protein structure and dynamics probed by MicroED.Biochemical Society transactions · 2026Review
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Strategies for mitigating radiation damage and improving data completeness in 3D electron diffraction of protein crystals.Acta crystallographica. Section D, Structural biology · 2026Article
- Review
- Article
- Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method.Structure (London, England : 1993) · 2023Review
- MicroED Structure of a Protoglobin Reactive Carbene Intermediate.Journal of the American Chemical Society · 2023Article
- Electron Diffraction of 3D Molecular Crystals.Chemical reviews · 2022Review
- A general method for directly phasing diffraction data from high-solvent-content protein crystals.IUCrJ · 2022Article
- Ab initio phasing macromolecular structures using electron-counted MicroED data.Nature methods · 2022Article
- Radiation damage to biological samples: still a pertinent issue.Journal of synchrotron radiation · 2021Article
- Establishing electron diffraction in chemical crystallography.Nature reviews. Chemistry · 2021Review
- An Overview of Microcrystal Electron Diffraction (MicroED).Annual review of biochemistry · 2021Review
- Studying Membrane Protein Structures by MicroED.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Folding of an Intrinsically Disordered Iron-Binding Peptide in Response to Sedimentation Revealed by Cryo-EM.Journal of the American Chemical Society · 2020Article
- Beyond protein structure determination with MicroED.Current opinion in structural biology · 2020Review
- Fragment-based determination of a proteinase K structure from MicroED data using ARCIMBOLDO_SHREDDER.Acta crystallographica. Section D, Structural biology · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present an example of an experimentally phased structure using only MicroED data. The structure of a seven-residue peptide is solved starting from differences to the diffraction intensities induced by structural changes due to radiation damage. The same wedge of reciprocal space was recorded twice by continuous-rotation MicroED from a set of 11 individual crystals. The data from the first pass were merged to make a "low-dose dataset." The data from the second pass were similarly merged to form a "damaged dataset." Differences between these two datasets were used to identify a single heavy-atom site from a Patterson difference map, and initial phases were generated. Finally, the structure was completed by iterative cycles of modeling and refinement.
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