Evidence map›Paper›PMID 32023434›Full record

ArticleBiophysical journal2020

Nanosecond-Timescale Dynamics and Conformational Heterogeneity in Human GCK Regulation and Disease.

Shawn M Sternisha, A Carl Whittington, Juliana A Martinez Fiesco, Carol Porter, Malcolm M McCray, Timothy Logan, Cristina Olivieri, Gianluigi Veglia, Peter J Steinbach, Brian G Miller

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 21 citations in OpenAlex.

  1. Investigation of structural and dynamic properties of the Butyrophilin BTN3A1/BTN2A1 cytoplasmic complex byFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
    Article
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  4. Concanavalin A Delivers a Photoactive Protein to the Bacterial Wall.International journal of molecular sciences · 2024
    Article
  5. Article
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  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Shawn M SternishaDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida.
A Carl WhittingtonDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida; Department of Biological Science, Florida State University, Tallahassee, Florida.
Juliana A Martinez FiescoDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida.
Carol PorterDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida.
Malcolm M McCrayDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida.
Timothy LoganDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida; Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida.
Cristina OlivieriDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota.
Gianluigi VegliaDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota; Department of Chemistry, University of Minnesota, Minneapolis, Minnesota.
Peter J SteinbachCenter for Molecular Modeling, Center for Information Technology, National Institutes of Health, Bethesda, Maryland. Electronic address: peter.steinbach@nih.gov.
Brian G MillerDepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida. Electronic address: bgmiller@fsu.edu.
Florida State University · USUniversity of Minnesota · USNational Institutes of Health · US

Funding

Mapping Allosteric Cooperativity in Protein KinasesR01GM100310 · NIGMS · UNIVERSITY OF MINNESOTA · PI VEGLIA, GIANLUIGI · 2012 to 2019
$4.3M
Enzyme Regulation by Intrinsic DynamicsR01GM115388 · NIGMS · FLORIDA STATE UNIVERSITY · PI MILLER, BRIAN GENE · 2016 to 2019
$1.4M
Evolutionary Origins of Protein RegulationR01GM133843 · NIGMS · FLORIDA STATE UNIVERSITY · PI MILLER, BRIAN GENE · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM100310NIGMS NIH HHS R01 GM115388NIGMS NIH HHS R01 GM133843
6 · The paper itself

Abstract

Human glucokinase (GCK) is the prototypic example of an emerging class of proteins with allosteric-like behavior that originates from intrinsic polypeptide dynamics. High-resolution NMR investigations of GCK have elucidated millisecond-timescale dynamics underlying allostery. In contrast, faster motions have remained underexplored, hindering the development of a comprehensive model of cooperativity. Here, we map nanosecond-timescale dynamics and structural heterogeneity in GCK using a combination of unnatural amino acid incorporation, time-resolved fluorescence, and

Indexed as

GlucokinaseHumansKineticsMagnetic Resonance SpectroscopyMolecular ConformationMutationGlucokinase

Identifiers

PMID32023434
PMCPMC7063420
OpenAlexW3000309743

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.