ArticleMolecular biology of the cell2020
Quantifying topography-guided actin dynamics across scales using optical flow.
Article in Molecular biology of the cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- OpticalFlow3D - a tool for measuring amorphous motion in three-dimensional fluorescence microscopy images.Journal of cell science · 2026Article
- Membrane Kymograph Generator: a cross-platform GUI software for automated generation and analysis of kymographs along dynamic cell boundaries.Bioinformatics (Oxford, England) · 2026Article
- Estradiol alters actin and protrusion dynamics in endometriotic epithelial cells.Journal of biological physics · 2026Article
- Membrane Kymograph Generator: A cross-platform GUI software for automated generation and analysis of kymographs along dynamic cell boundaries.bioRxiv : the preprint server for biology · 2026Article
- Cellular Responses to Nanoscale Topography Mediated Through the RhoA/ROCK Pathway.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Nascent actin dynamics and the disruption of calcium dynamics by actin arrest in developing neural cell networks.Communications biology · 2025Article
- Interactions between CNS regulation and serotonergic modulation of crayfish hindgut motility.Royal Society open science · 2025Article
- Spatiotemporal feature learning for actin dynamics.PloS one · 2025Article
- Nanotopographic control of actin waves and growth cone navigation in developing neurons.Frontiers in cell and developmental biology · 2025Article
- CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnover.Molecular biology of the cell · 2024Article
- A minimal physical model for curvotaxis driven by curved protein complexes at the cell's leading edge.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Coupling of Perinuclear Actin Cap and Nuclear Mechanics in Regulating Flow-Induced Yap Spatiotemporal Nucleocytoplasmic Transport.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- A dynamic partitioning mechanism polarizes membrane protein distribution.Nature communications · 2023Article
- CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnover.bioRxiv : the preprint server for biology · 2023Article
- Quantitative structured illumination microscopy via a physical model-based background filtering algorithm reveals actin dynamics.Nature communications · 2023Article
- Nanotopography modulates intracellular excitable systems through cytoskeleton actuation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Self-supervised machine learning for live cell imagery segmentation.Communications biology · 2022Article
- Nanotopography modulates cytoskeletal organization and dynamics during T cell activation.Molecular biology of the cell · 2022Article
- Bidirectional feedback between BCR signaling and actin cytoskeletal dynamics.The FEBS journal · 2022Review
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Authors and funding
7 authors.
Funding
Abstract
The dynamic rearrangement of the actin cytoskeleton is an essential component of many mechanotransduction and cellular force generation pathways. Here we use periodic surface topographies with feature sizes comparable to those of in vivo collagen fibers to measure and compare actin dynamics for two representative cell types that have markedly different migratory modes and physiological purposes: slowly migrating epithelial MCF10A cells and polarizing, fast-migrating, neutrophil-like HL60 cells. Both cell types exhibit reproducible guidance of actin waves (esotaxis) on these topographies, enabling quantitative comparisons of actin dynamics. We adapt a computer-vision algorithm, optical flow, to measure the directions of actin waves at the submicron scale. Clustering the optical flow into regions that move in similar directions enables micron-scale measurements of actin-wave speed and direction. Although the speed and morphology of actin waves differ between MCF10A and HL60 cells, the underlying actin guidance by nanotopography is similar in both cell types at the micron and submicron scales.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.