ArticleThe ISME journal2020
Plasmids persist in a microbial community by providing fitness benefit to multiple phylotypes.
Article in The ISME journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 89 citations in OpenAlex.
- Plasmid-Host Networks Reveal Persistent Mobile Elements Across Wastewater Treatment.Nature communications · 2026Article
- Invasive plasmids as ecosystem engineers-from mechanism to application.Essays in biochemistry · 2026Review
- Biofilm-forming traits enrich the plasmid diversity and functional potential in particle-attached bacteria in coastal ecosystems.Microbiology spectrum · 2026Article
- Climate change and the global spread of antimicrobial resistance in livestock systems: a comprehensive review.One health outlook · 2026Review
- Beyond borders: plasmids drive a shared antibiotic resistome in European urban water systems.Microbiome · 2026Article
- Genomic Insights into the Probiotic Functionality and Safety ofFoods (Basel, Switzerland) · 2025Article
- Interactions and evolutionary relationships among bacterial mobile genetic elements.Nature reviews. Microbiology · 2025Review
- The metabolic burden associated with plasmid acquisition: An assessment of the unrecognized benefits to host cells.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Fitness and adaptive evolution of a Rhodococcus sp. harboring dioxin-catabolic plasmids.World journal of microbiology & biotechnology · 2025Article
- An eco-evolutionary perspective on antimicrobial resistance in the context of One Health.iScience · 2025Review
- Food Webs and Feedbacks: The Untold Ecological Relevance of Antimicrobial Resistance as Seen in Harmful Algal Blooms.Microorganisms · 2024Review
- Single-cell RNA sequencing reveals plasmid constrains bacterial population heterogeneity and identifies a non-conjugating subpopulation.Nature communications · 2024Article
- Plasmid-Borne Biosynthetic Gene Clusters within a Permanently Stratified Marine Water Column.Microorganisms · 2024Article
- Antibiotic resistance begets more resistance: chromosomal resistance mutations mitigate fitness costs conferred by multi-resistant clinical plasmids.Microbiology spectrum · 2024Article
- A generic approach to infer community-level fitness of microbial genes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Biodiversity of carbapenem-resistant bacteria in clinical samples from the Southwest Amazon region (Rondônia/Brazil).Scientific reports · 2024Article
- Global epistasis in plasmid-mediated antimicrobial resistance.Molecular systems biology · 2024Review
- Conjugal plasmid transfer in the plant rhizosphere in the One Health context.Frontiers in microbiology · 2024Article
- Plasmids, a molecular cornerstone of antimicrobial resistance in the One Health era.Nature reviews. Microbiology · 2024Review
- Antimicrobial resistance level and conjugation permissiveness shape plasmid distribution in clinical enterobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current epidemic of antibiotic resistance has been facilitated by the wide and rapid horizontal dissemination of antibiotic resistance genes (ARGs) in microbial communities. Indeed, ARGs are often located on plasmids, which can efficiently shuttle genes across diverse taxa. While the existence conditions of plasmids have been extensively studied in a few model bacterial populations, their fate in complex bacterial communities is poorly understood. Here, we coupled plasmid transfer assays with serial growth experiments to investigate the persistence of the broad-host-range IncP-1 plasmid pKJK5 in microbial communities derived from a sewage treatment plant. The cultivation conditions combined different nutrient and oxygen levels, and were non-selective and non-conducive for liquid-phase conjugal transfer. Following initial transfer, the plasmid persisted in almost all conditions during a 10-day serial growth experiment (equivalent to 60 generations), with a transient transconjugant incidence up to 30%. By combining cell enumeration and sorting with amplicon sequencing, we mapped plasmid fitness effects across taxa of the microbial community. Unexpected plasmid fitness benefits were observed in multiple phylotypes of Aeromonas, Enterobacteriaceae, and Pseudomonas, which resulted in community-level plasmid persistence. We demonstrate, for the first time, that plasmid fitness effects across community members can be estimated in high-throughput without prior isolation. By gaining a fitness benefit when carrying plasmids, members within complex microbial communities might have a hitherto unrecognised potential to maintain plasmids for long-term community-wide access.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.