Evidence map›Paper›PMID 32019988›Full record

ArticleScientific reports2020

Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy.

Deepak Bhere, Nahid Arghiani, Esther Revai Lechtich, Yizheng Yao, Sarah Alsaab, Fengfeng Bei, Maryam M Matin, Khalid Shah

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 41 citations in OpenAlex.

  1. Review
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  7. Article
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  9. Review
  10. miRNA-Based Technologies in Cancer Therapy.Journal of personalized medicine · 2023
    Review
  11. Optimal delivery of RNA interference by viral vectors for cancer therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2023
    Review
  12. Article
  13. Role of MicroRNA-7 (MiR-7) in Cancer Physiopathology.International journal of molecular sciences · 2022
    Review
  14. Review
  15. Review
  16. Review
  17. An Assessment on Ethanol-Blended Gasoline/Diesel Fuels on Cancer Risk and Mortality.International journal of environmental research and public health · 2021
    Review
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  19. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Deepak Bhere *Center for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Nahid Arghiani *Center for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Esther Revai LechtichCenter for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Yizheng YaoDepartment of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Sarah AlsaabCenter for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Fengfeng BeiDepartment of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
Maryam M MatinDepartment of Biology and Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Khalid ShahCenter for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA. kshah@bwh.harvard.edu.
Harvard University · USBrigham and Women's Hospital · USFerdowsi University of Mashhad · IR

Funding

In vivo imaging of the stem cell loaded oncolytic viruses for cancer therapyR01CA204720 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI SHAH, KHALID A · 2016 to 2020
$1.9M
NCI NIH HHS R01 CA204720
6 · The paper itself

Abstract

Dysregulation of miRNA expression has been implicated in cancer. Numerous strategies have been explored to modulate miR but sub-optimal delivery and inability to concurrently target multiple pathways involved in tumor progression have limited their efficacy. In this study, we explored the potential co-modulation of upregulated miR-21 and downregulated miR-7 to enhance therapeutic outcomes in heterogenic tumor types. We first engineered lentiviral (LV) and adeno-associated viral (AAV) vectors that preferentially express anti-sense miR against miR-21(miRzip-21) and show that modulating miR-21 via miRzip extensively targets tumor cell proliferation, migration and invasion in vitro in a broad spectrum of cancer types and has therapeutic efficacy in vivo. Next, we show a significantly increased expression of caspase-mediated apoptosis by simultaneously downregulating miR-21 and upregulating miR-7 in different tumor cells. In vivo co-treatment with AAV-miRzip-21 and AAV-miR-7 in mice bearing malignant brain tumors resulted in significantly decreased tumor burden with a corresponding increase in survival. To our knowledge, this is the first study that demonstrates the therapeutic efficacy of simultaneously upregulating miR-7 and downregulating miR-21 and establishes a roadmap towards clinical translation of modulating miRs for various cancer types.

Indexed as

Down-RegulationUp-RegulationAnimalsApoptosisCell Line, TumorCell MovementCell ProliferationCell SurvivalGene Expression Regulation, NeoplasticGlioblastomaHumansMesenchymal Stem CellsMiceMicroRNAsXenograft Model Antitumor AssaysMicroRNAsMIRN21 microRNA, humanMIRN7-1 microRNA, human

Identifiers

PMID32019988
PMCPMC7000780
OpenAlexW3005500985

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.