Evidence map›Paper›PMID 32019904›Full record

ArticleAging2020

Long non-coding RNA NNT-AS1 promotes cholangiocarcinoma cells proliferation and epithelial-to-mesenchymal transition through down-regulating miR-203.

Yulei Gu, Zhiqiang Zhu, Hui Pei, Dong Xu, Yumin Jiang, Luanluan Zhang, Lili Xiao

RetractedOpen access · greenAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Long Non-Coding RNAs as Molecular Biomarkers in Cholangiocarcinoma.Frontiers in cell and developmental biology · 2022
    Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Overexpression of NNT-AS1 Activates TGF-BioMed research international · 2020
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yulei GuEmergency Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Zhiqiang ZhuEmergency Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Hui PeiEmergency Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Dong XuEmergency Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Yumin JiangEmergency Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Luanluan ZhangEmergency Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Lili XiaoDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
First Affiliated Hospital of Zhengzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCholangiocarcinoma (CCA) is a serious malignant tumor. Long non-coding RNA NNT-AS1 (NNT-AS1) takes crucial roles in several tumors. So, we planned to research the roles and underlying mechanism of NNT-AS1 in CCA.

resultsNNT-AS1 overexpression was appeared in CCA tissues and cell lines. Proliferation was promoted by NNT-AS1 overexpression in CCLP1 and TFK1 cells. Besides, NNT-AS1 overexpression reduced E-cadherin level and raised levels of N-cadherin, vimentin, Snail and Slug. However, the opposite trend was occurred by NNT-AS1 knockdown. Further, NNT-AS1 overexpression promoted phosphatidylinositol 3 kinase (PI3K)/AKT and extracellular signal-regulated kinase (ERK)1/2 pathways. MiR-203 was sponged by NNT-AS1 and miR-203 mimic reversed the above promoting effects of NNT-AS1. Additionally, insulin-like growth factor type 1 receptor (IGF1R) and zinc finger E-box binding homeobox 1 (ZEB1) were two potential targets of miR-203.

conclusionNNT-AS1 promoted proliferation, EMT and PI3K/AKT and ERK1/2 pathways in CCLP1 and TFK1 cells through down-regulating miR-203.

methodsCCLP1 and TFK1 cells were co-transfected with pcDNA-NNT-AS1 and miR-203 mimic. Bromodeoxyuridine (BrdU), flow cytometry, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot were employed to detect roles and mechanism of NNT-AS1. Interaction between NNT-AS1 and miR-203 or miR-203 and target genes was examined through luciferase activity experiment.

Indexed as

Antigens, CDBile Duct NeoplasmsCadherinsCell Line, TumorCell ProliferationCholangiocarcinomaDown-RegulationEpithelial-Mesenchymal TransitionGene Knockdown TechniquesGene Knock-In TechniquesHumansMAP Kinase Signaling SystemMicroRNAsMitochondrial ProteinsNADP Transhydrogenase, AB-SpecificPhosphatidylinositol 3-KinasesAntigens, CDCadherinsCDH1 protein, humanCDH2 protein, humanMicroRNAsMIRN203 microRNA, humanMitochondrial ProteinsNADP Transhydrogenase, AB-SpecificNNT protein, humanPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktReceptor, IGF Type 1RNA, AntisenseRNA, Long NoncodingSNAI1 protein, humanSnail Family Transcription FactorsVimentinZEB1 protein, humanZinc Finger E-box-Binding Homeobox 1cholangiocarcinomaepithelial-to-mesenchymal transitionlong non-coding RNA NNT-AS1miR-203proliferation

Identifiers

PMID32019904
PMCPMC7041725
OpenAlexW3005233185

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.