Evidence map›Paper›PMID 32019566›Full record

ArticleMolecular cancer2020

Long non-coding RNA SOX2OT promotes the stemness phenotype of bladder cancer cells by modulating SOX2.

Yonghao Zhan, Zhicong Chen, Shiming He, Yanqing Gong, Anbang He, Yifan Li, Lianghao Zhang, Xuepei Zhang, Dong Fang, Xuesong Li and 1 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 77 papers.

0numbers the graph read from it
0cells of the map it votes in
77citing papers in PubMed
8.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

77 citing papers in PubMed, 147 citations in OpenAlex.

  1. Review
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  6. Small extracellular vesicles (sEVs) in pancreatic cancer progression and diagnosis.Journal of controlled release : official journal of the Controlled Release Society · 2025
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  20. [LncRNA SOX2OT enhances 5-fluorouracil resistance of cholangiocarcinoma cells by promoting autophagyNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2024
    Article

17 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Yonghao Zhan *Department of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China.ORCID http://orcid.org/0000-0003-1282-2637
Zhicong Chen *Department of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China.
Shiming He *Department of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China.
Yanqing Gong *Department of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China.
Anbang HeDepartment of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China.
Yifan LiDepartment of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China.
Lianghao ZhangDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450003, China.
Xuepei ZhangDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450003, China.
Dong FangDepartment of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China. fdmailbox@126.com.
Xuesong LiDepartment of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China. pineneedle@sina.com.
Liqun ZhouDepartment of Urology, Peking University First Hospital, The Institute of Urology, Peking University, National Urological Cancer Center, No. 8 Xishiku street, Beijing, 100034, China. zhoulqmail@sina.com.
Peking University · CNFirst Affiliated Hospital of Zhengzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAccumulating evidence indicates that long non-coding RNAs (lncRNAs) are potential biomarkers and key regulators of tumour development and progression. SOX2 overlapping transcript (SOX2OT) is a novel lncRNA that acts as a potential biomarker and is involved in the development of cancer and cancer stem cells. However, the clinical significance and molecular mechanism of SOX2OT in bladder cancer are still unknown.

methodsThe expression level of SOX2OT was determined by RT-qPCR in a total of 106 patients with urothelial bladder cancer and in different bladder cancer cell (BCC) lines. Bladder cancer stem cells (BCSCs) were isolated from BCCs using flow cytometry based on the stem cell markers CD44 and ALDH1. Loss-of-function experiments were performed to investigate the biological roles of SOX2OT in the stemness phenotype of BCSCs. Comprehensive transcriptional analysis, RNA FISH, dual-luciferase reporter assays and western blots were performed to explore the molecular mechanisms underlying the functions of SOX2OT.

resultsSOX2OT was highly expressed in bladder cancer, and increased SOX2OT expression was positively correlated with a high histological grade, advanced TNM stage and poor prognosis. Further experiments demonstrated that knockdown of SOX2OT inhibited the stemness phenotype of BCSCs. Moreover, inhibition of SOX2OT delayed xenograft tumour growth and decreased metastases in vivo. Mechanistically, we found that SOX2OT was mainly distributed in the cytoplasm and positively regulated SOX2 expression by sponging miR-200c. Furthermore, SOX2 overexpression reversed the SOX2OT silencing-induced inhibition of the BCSC stemness phenotype.

conclusionThis study is the first to demonstrate that SOX2OT plays an important regulatory role in BCSCs and that SOX2OT may serve as a potential diagnostic biomarker and therapeutic target in bladder cancer.

Indexed as

Gene Expression Regulation, NeoplasticAnimalsApoptosisBiomarkers, TumorCell ProliferationFemaleHumansMaleMiceMice, Inbred BALB CMice, NudeMicroRNAsMiddle AgedNeoplastic Stem CellsPrognosisRNA, Long NoncodingBiomarkers, Tumorlong non-coding RNA Sox2ot, humanMicroRNAsMIRN200 microRNA, humanRNA, Long NoncodingSOX2 protein, humanSOXB1 Transcription FactorsBladder cancerCancer stem cellmiR-200cSOX2SOX2OT

Identifiers

PMID32019566
PMCPMC6998848
OpenAlexW3008805691

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.