ArticleViruses2020
Lyophilized Matrix Containing Ready-to-Use Primers and Probe Solution for Standardization of Real-Time PCR and RT-qPCR Diagnostics in Virology.
Article in Viruses, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Aetiology of acute respiratory infection in Vientiane, Lao PDR, from a case-control study.Scientific reports · 2026Article
- Evaluation of the Novel RITA MTBC Assay for Tuberculosis Detection: A Pilot Comparison with GeneXpert and BD MAX™.Pathogens (Basel, Switzerland) · 2025Article
- Development and evaluation of a duplex RT-qPCR assay for the detection and identification of Mayaro and chikungunya viruses.Journal of clinical microbiology · 2025Article
- Pneumococcal density and respiratory co-detection in severe pediatric pneumonia in Laos.Scientific reports · 2025Article
- External quality assessment for molecular detection of sand fly-borne phleboviruses circulating in the Mediterranean Basin.Parasites & vectors · 2025Article
- Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR.PloS one · 2025Article
- Dry Swabs and Dried Saliva as Alternative Samples for SARS-CoV-2 Detection in Remote Areas in Lao PDR.Open forum infectious diseases · 2024Article
- Seroprevalence study in humans and molecular detection in Rhipicephalus sanguineus ticks of severe fever with thrombocytopenia syndrome virus in Thailand.Scientific reports · 2024Article
- Diversity and Genetic Reassortment of Keystone Virus in Mosquito Populations in Florida.The American journal of tropical medicine and hygiene · 2023Article
- Evaluation of a novel lyophilized-pellet-based 2019-nCoV nucleic acid detection kit for the diagnosis of COVID-19.PloS one · 2023Article
- Genome Sequence of Genotype 1A Hepatovirus A Isolated from Plasma from a Haitian Child.Microbiology resource announcements · 2022Article
- A clinical, aetiological, and public health perspective on central nervous system infections in Bolivia, 2017-2018.Scientific reports · 2021Article
- Transferable, easy-to-use and room-temperature-storable PCR mixes for microfluidic molecular diagnostics.Talanta · 2021Article
- Lyophilization of premixed COVID-19 diagnostic RT-qPCR reactions enables stable long-term storage at elevated temperature.Biotechnology journal · 2021Article
- Screening of Additive Formulations Enables Off-Chip Drop Reverse Transcription Quantitative Polymerase Chain Reaction of Single Influenza A Virus Genomes.Analytical chemistry · 2021Article
- Evaluation of a Trio Toscana Virus Real-Time RT-PCR Assay Targeting Three Genomic Regions within Nucleoprotein Gene.Pathogens (Basel, Switzerland) · 2021Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time molecular techniques have become the reference methods for direct diagnosis of pathogens. The reduction of steps is a key factor in order to decrease the risk of human errors resulting in invalid series and delayed results. We describe here a process of preparation of oligonucleotide primers and hydrolysis probe in a single tube at predefined optimized concentrations that are stabilized via lyophilization (Lyoph-P&P). Lyoph-P&P was compared versus the classic protocol using extemporaneously prepared liquid reagents using (i) sensitivity study, (ii) long-term stability at 4 °C, and (iii) long-term stability at 37 °C mimicking transportation without cold chain. Two previously published molecular assays were selected for this study. They target two emerging viruses that are listed on the blueprint of the WHO as to be considered for preparedness and response actions: chikungunya virus (CHIKV) and Rift Valley fever phlebovirus (RVFV). Results of our study demonstrate that (i) Lyoph-P&P is stable for at least 4 days at 37 °C supporting shipping without the need of cold chain, (ii) Lyoph-P&P rehydrated solution is stable at +4 °C for at least two weeks, (iii) sensitivity observed with Lyoph-P&P is at least equal to, often better than, that observed with liquid formulation, (iv) validation of results observed with low-copy specimens is rendered easier by higher fluorescence level. In conclusion, Lyoph-P&P holds several advantages over extemporaneously preparer liquid formulation that merit to be considered when a novel real-time molecular assay is implemented in a laboratory in charge of routine diagnostic activity.
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