Evidence map›Paper›PMID 32017920›Full record

ArticleCell chemical biology2020

A Genetic Toggle for Chemical Control of Individual Plk1 Substrates.

James M Johnson, Alexander S Hebert, Quentin H Drane, Robert F Lera, Jun Wan, Beth A Weaver, Joshua J Coon, Mark E Burkard

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

James M JohnsonDepartment of Medicine, Division of Hematology/Oncology, University of Wisconsin, 1111 Highland Avenue, WIMR 6059, Madison, WI 53705, USA; University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA.
Alexander S HebertGenome Center, University of Wisconsin, Madison, WI 53705, USA; Morgridge Institute for Research, Madison, WI 53705, USA.
Quentin H DraneDepartment of Medicine, Division of Hematology/Oncology, University of Wisconsin, 1111 Highland Avenue, WIMR 6059, Madison, WI 53705, USA; University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA.
Robert F LeraDepartment of Medicine, Division of Hematology/Oncology, University of Wisconsin, 1111 Highland Avenue, WIMR 6059, Madison, WI 53705, USA; University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA.
Jun WanPhysiology Training Program, University of Wisconsin, Madison, WI 53705, USA.
Beth A WeaverUniversity of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA; Department of Cell and Regenerative Biology, University of Wisconsin, Madison, WI 53705, USA; Department of Oncology/McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53705, USA.
Joshua J CoonGenome Center, University of Wisconsin, Madison, WI 53705, USA; Department of Chemistry, University of Wisconsin, Madison, WI 53705, USA; Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53705, USA; Morgridge Institute for Research, Madison, WI 53705, USA.
Mark E BurkardDepartment of Medicine, Division of Hematology/Oncology, University of Wisconsin, 1111 Highland Avenue, WIMR 6059, Madison, WI 53705, USA; University of Wisconsin Carbone Cancer Center, University of Wisconsin, Madison, WI 53705, USA; Department of Oncology/McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53705, USA. Electronic address: mburkard@wisc.edu.
University of Wisconsin–Madison · US

Funding

UW COMPREHENSIVE CANCER CENTER SUPPORTP30CA014520 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI AMY TRENTHAM-DIETZ · 1985 to 2026
$142.6M
Viral VectorP30CA086862 · NCI · UNIVERSITY OF IOWA · PI Douglas Robert Spitz · 2000 to 2026
$70.0M
TR&D 2 Metabolic Labels for Ultraplexed Protein Quantification p. 453P41GM108538 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J · 2016 to 2025
$13.1M
Structure, Function and Regulation of the ProteomeR35GM118110 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI COON, JOSHUA J · 2016 to 2025
$9.1M
Graduate Training In Cellular And Molecular Pathogenesis Of Human DiseasesT32GM081061 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI FABRY, ZSUZSANNA · 2010 to 2019
$1.8M
Separation of late mitotic functions of polo-like kinase 1 with chemical geneticsR01GM097245 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BURKARD, MARK E · 2011 to 2015
$1.4M
NCI NIH HHS P30 CA014520NCI NIH HHS P30 CA086862NIGMS NIH HHS P41 GM108538NIGMS NIH HHS R01 GM097245NIGMS NIH HHS R35 GM118110NIGMS NIH HHS T32 GM081061
6 · The paper itself

Abstract

Polo-like kinase 1 has hundreds of substrates and multiple functions that operate within the ∼60 min of mitosis. Herein, we describe a chemical-genetic system that allows particular substrates to be "toggled" into or out of chemical control using engineered phosphoacceptor selectivity. Biochemical assays and phosphoproteomic analysis of mitotic cell extracts showed that Plk1

Indexed as

Cell Cycle ProteinsHumansMitosisPhosphorylationPolo-Like Kinase 1Protein EngineeringProtein Serine-Threonine KinasesProto-Oncogene ProteinsSerineSubstrate SpecificityThreonineCell Cycle ProteinsPolo-Like Kinase 1Protein Serine-Threonine KinasesProto-Oncogene ProteinsSerineThreoninechemical biologychemical-geneticsgenetic toggleKif2bkinasemitosisphosphoacceptor selectivityphosphorylation

Identifiers

PMID32017920
PMCPMC7239509
OpenAlexW3004375738

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.