ArticlePloS one2020
Reference gene selection for quantitative real-time PCR (qRT-PCR) expression analysis in Galium aparine L.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
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- Identification and validation of qRT-PCR reference genes for analyzing grape infection with gray mold.BMC genomics · 2024Article
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- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Integrated omic techniques and their genomic features for invasive weeds.Functional & integrative genomics · 2023Review
- Validation of suitable reference genes by various algorithms for gene expression analysis in Isodon rubescens under different abiotic stresses.Scientific reports · 2022Article
- Brain Proteome and Behavioural Analysis in Wild Type, BDNFInternational journal of molecular sciences · 2022Article
- Identification of suitable reference genes for quantitative reverse transcription PCR inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Expression Pattern of Entire Cytochrome P450 Genes and Response of Defenses in a Metabolic-Herbicide-Resistant Biotype ofFrontiers in plant science · 2022Article
- The analysis of reference genes expression stability in susceptible and resistant Apera spica-venti populations under herbicide treatment.Scientific reports · 2021Article
- Validation of Reference Genes for Quantitative PCR in Johnsongrass (Plants (Basel, Switzerland) · 2021Article
- Positive feedback regulation between USP15 and ERK2 inhibits osteoarthritis progression through TGF-β/SMAD2 signaling.Arthritis research & therapy · 2021Article
- Reference gene and tropomyosin expression in mud crab Scylla olivacea, Scylla paramamosain and Scylla tranquebarica.Molecular biology reports · 2020Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To accurately evaluate expression levels of target genes, stable internal reference genes is required for normalization of quantitative real-time PCR (qRT-PCR) data. However, there have been no systematical investigation on the stability of reference genes used in the bedstraw weed, Galium aparine L. (BGA). In this study, the expression profiles of seven traditionally used reference genes, namely 18S, 28S, ACT, GAPDH, EF1α, RPL7 and TBP in BGA were assessed under both biotic (developmental time and tissue), and abiotic (temperature, regions and herbicide) conditions. Four analytical algorithms (geNorm, Normfinder, BestKeeper and the ΔCt method) were used to analyze the suitability of these genes as internal reference genes. RefFinder, a comprehensive analytical software, was used to rank the overall stability of the candidate genes. The optimal normalization internal control genes were ranked as: 28S and RPL7 were best for all the different experimental conditions (developmental stages, tissues, temperature, regions and herbicide treatment); 28S and RPL7 for developmental stages; TBP and GAPDH for different tissues; 28S and GAPDH were relatively stable for different temperature; 28S and TBP were suitable for herbicide treatment. A specific set of reference genes were recommended for each experimental condition in BGA.
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