Evidence map›Paper›PMID 32016855›Full record

ArticlePharmacological reports : PR2020

Effect of 3-O-acetylaleuritolic acid from in vitro-cultured Drosera spatulata on cancer cells survival and migration.

Ewa Toton, Izabela Kedziora, Aleksandra Romaniuk-Drapala, Natalia Konieczna, Mariusz Kaczmarek, Natalia Lisiak, Anna Paszel-Jaworska, Anna Rybska, Wiktoria Duszynska, Jaromir Budzianowski and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Pharmacological reports : PR, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Stemness-Suppressive Effect of Bibenzyl fromEvidence-based complementary and alternative medicine : eCAM · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Ewa TotonDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland. etoton@ump.edu.pl.
Izabela KedzioraDepartment of Pharmaceutical Botany, Poznan University of Medical Sciences, Marii Magdaleny 14, 61-861, Poznan, Poland.
Aleksandra Romaniuk-DrapalaDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.
Natalia KoniecznaDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.
Mariusz KaczmarekDepartment of Clinical Immunology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806, Poznan, Poland.
Natalia LisiakDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.
Anna Paszel-JaworskaDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.
Anna RybskaPoznan University of Medical Sciences, Poznan, Poland.
Wiktoria DuszynskaPoznan University of Medical Sciences, Poznan, Poland.
Jaromir BudzianowskiDepartment of Pharmaceutical Botany, Poznan University of Medical Sciences, Marii Magdaleny 14, 61-861, Poznan, Poland.
Maria RybczynskaDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.
Blazej RubisDepartment of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Przybyszewskiego 49, 60-355, Poznan, Poland.
Poznan University of Medical Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDrosera spatulata is a source of many compounds such as naphthoquinones, phenolic acids, flavonoids, anthocyanins, and naphthalene derivatives. Unfortunately, the information regarding the biological activity and chemical profile of those compounds is still incomplete. Herein, we investigated the biological activity of 3-O-acetylaleuritolic acid (3-O-AAA) in cancer cell lines.

methodsThe cell viability of HeLa, HT-29, MCF7, and MCF12A cells was assessed using MTT assay. Proliferation potential was assessed using the clonogenic assay and flow cytometry. Migration modulation was tested using a scratch assay. Protein expression was analyzed by immunoblotting.

results3-O-AAA significantly inhibited the growth of all tested tumor cells. The results of the colony formation assay suggested cytostatic properties of the studied compound. The scratch assay showed that 3-O-AAA was an efficient migration inhibitor in a dose-dependent manner. Moreover, it caused modulation of mTOR, beclin1, and Atg5 proteins suggesting a possible role of the compound in autophagy induction.

conclusionCollectively, these results demonstrated that 3-O-AAA inhibited the proliferation and migration of cancer cell lines as well as contributed to autophagy induction showing some anticancer properties.

Indexed as

Antineoplastic Agents, PhytogenicAutophagyCell MovementCell ProliferationCell SurvivalDose-Response Relationship, DrugDroseraHeLa CellsHT29 CellsHumansMCF-7 CellsPalmitic Acids3-O-acetyl aleuritolic acidAntineoplastic Agents, PhytogenicPalmitic Acids3-O-acetylaleuritolic acidAutophagyDrosera spatulataMigration

Identifiers

PMID32016855
OpenAlexW3000031430

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.