ArticleRice (New York, N.Y.)2020
Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing.
Article in Rice (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Advances in CRISPR/Cas systems for engineering abiotic stress tolerance in plants: mechanisms and future prospects.Planta · 2026Review
- Strategies to develop climate-resilient chili peppers: transcription factor optimization through genome editing.Planta · 2025Review
- Validation of CRISPR construct activity and gene function in melon via a hairy root transformation system.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Optimization of CRISPR-Cas9 system iniScience · 2024Article
- An efficient CRISPR-Cas12a promoter editing system for crop improvement.Nature plants · 2023Article
- Building Blocks of Artificial CRISPR-Based Systems beyond Nucleases.International journal of molecular sciences · 2022Review
- CRISPR/Cas9 in Planta Hairy Root Transformation: A Powerful Platform for Functional Analysis of Root Traits in Soybean.Plants (Basel, Switzerland) · 2022Review
- CRISPR-BETS: a base-editing design tool for generating stop codons.Plant biotechnology journal · 2022Article
- CRISPR-Cas9 mediated OsMIR168a knockout reveals its pleiotropy in rice.Plant biotechnology journal · 2022Article
- Establishment of an Efficient Genome Editing System in Lettuce Without Sacrificing Specificity.Frontiers in plant science · 2022Article
- Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations.Frontiers in plant science · 2022Review
- Improved plant cytosine base editors with high editing activity, purity, and specificity.Plant biotechnology journal · 2021Article
- Article
- Efficient deletion of multiple circle RNA loci by CRISPR-Cas9 reveals Os06circ02797 as a putative sponge for OsMIR408 in rice.Plant biotechnology journal · 2021Article
- Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems.Nature communications · 2021Article
- Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters.Horticulture research · 2021Article
- PAM-less plant genome editing using a CRISPR-SpRY toolbox.Nature plants · 2021Article
- Exploring C-To-G Base Editing in Rice, Tomato, and Poplar.Frontiers in genome editing · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundExpression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. In order to make this system routine in plant biology, engineering efforts are needed for developing and optimizing such single transcript unit (STU) systems for plant genome editing.
resultsTo develop novel intron-based STU (iSTU) CRISPR system (STU CRISPR 3.0), we first evaluated three introns from three plant species for carrying guide RNAs by using an enhanced green fluorescence protein (eGFP) system in rice. After validation of proper intron slicing, we inserted these gRNA-containing introns into the open reading frames (ORFs) of Cas9 and Cas12a for testing their genome editing capability. Different guide RNA processing strategies have been tested for Cas9 and Cas12a. We demonstrated singular genome editing and multiplexed genome editing with these iSTU-Cas9 and iSTU-Cas12a systems.
conclusionWe developed multiple iSTU-CRISPR/Cas9 and Cas12a systems for plant genome editing. Our results shed light on potential directions for further improvement of the iSTU systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.