Evidence map›Paper›PMID 32016474›Full record

ArticleMolecular medicine reports2020

Identification of HeLa cell proteins that interact with Chlamydia trachomatis glycogen synthase using yeast two‑hybrid assays.

Zhenjie Sun, Yuhui Sun, Yumeng Li, Xiuli Luan, Hui Chen, Haiying Wu, Bo Peng, Chunxue Lu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Prohibitins: A Key Link between Mitochondria and Nervous System Diseases.Oxidative medicine and cellular longevity · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zhenjie Sun *Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Yuhui Sun *Institution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Yumeng LiInstitution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Xiuli LuanInstitution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Hui ChenInstitution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Haiying WuInstitution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Bo PengDepartment of Pathology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
Chunxue LuInstitution of Pathogenic Biology, Hengyang Medical College, University of South China, Hengyang, Hunan 421001, P.R. China.
University of South China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chlamydia trachomatis (C. trachomatis) is the leading cause of bacterial sexually transmitted diseases and infectious diseases that cause blindness. The pathophysiology of chlamydial infections is poorly understood, but secreted proteins have emerged as key virulence factors. C. trachomatis glycogen synthase (GlgA) is a chlamydial secretory protein, which localizes in the lumen of chlamydial inclusion bodies and the cytosol of host cells. In order to improve understanding of the roles of GlgA in chlamydial pathogenesis, four proteins that interact with GlgA, Homo sapiens CXXC finger protein 1, prohibitin (PHB), gelsolin‑like actin‑capping protein and apolipoprotein A‑I binding protein were identified using yeast two‑hybrid assays. The functions of these proteins are complex, and preliminary results suggested that PHB interacts with GlgA. However, further studies are required to determine the specific interactions of these proteins with GlgA. The findings of the present study may provide a direction and foundation for future studies focusing on the mechanism of GlgA in C. trachomatis infection.

Indexed as

Protein Interaction MappingTwo-Hybrid System TechniquesBacterial ProteinsCarrier ProteinsChlamydia InfectionsChlamydia trachomatisGlycogen SynthaseHeLa CellsHost-Pathogen InteractionsHumansPlasmidsProhibitinsProtein BindingReproducibility of ResultsBacterial ProteinsCarrier ProteinsGlycogen SynthasePHB protein, humanProhibitinsChlamydia trachomatisglycogen synthaseyeast two-hybrid assay

Identifiers

PMID32016474
PMCPMC7003024
OpenAlexW2999463298

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.