ArticleMolecular medicine reports2020
New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation.
Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 23 citations in OpenAlex.
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- Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium).Journal of ovarian research · 2024Article
- Epigenetic-related transcriptional reprogramming elucidated by identification and validation of a novel reference gene combination for RT-qPCR studies in porcine oocytes of contrasting quality.Molecular biology reports · 2024Article
- KAS-seq profiling captures transcription dynamics during oocyte maturation.Journal of ovarian research · 2024Article
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- Whole-genome sequencing of cryopreserved resources from French Large White pigs at two distinct sampling times reveals strong signatures of convergent and divergent selection between the dam and sire lines.Genetics, selection, evolution : GSE · 2023Article
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- Bone morphogenetic protein 2- and estradiol-17β-induced changes in ovarian transcriptome during primordial follicle formation†.Biology of reproduction · 2022Article
- Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes.International journal of molecular sciences · 2022Article
- Beneficial Effects of Traditional Chinese Medicine in the Treatment of Premature Ovarian Failure.Evidence-based complementary and alternative medicine : eCAM · 2022Review
- Alpha-lipoic acid supplementation restores the meiotic competency and fertilization capacity of porcine oocytes induced by arsenite.Frontiers in cell and developmental biology · 2022Article
- Identification of Genomic Regions Influencing N-Metabolism and N-Excretion in Lactating Holstein- Friesians.Frontiers in genetics · 2021Article
- Pervasive 3'-UTR Isoform Switches During Mouse Oocyte Maturation.Frontiers in molecular biosciences · 2021Article
- Antioxidant Nobiletin Enhances Oocyte Maturation and Subsequent Embryo Development and Quality.International journal of molecular sciences · 2020Article
- The challenge of getting a high quality of RNA from oocyte for gene expression study.Veterinary research forum : an international quarterly journal · 2020Article
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Authors and funding
19 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well‑known, its exact molecular mechanisms remain widely undiscovered. Oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature has indicated that the oocyte itself serves a regulatory role in the mechanisms underlying these processes. Hence, the present study performed expression microarrays to analyze the complete transcriptome of porcine oocytes during their in vitro maturation (IVM). Pig material was used for experimentation, as it possesses similarities to the reproductive processes and general genetic proximities of Sus scrofa to human. Oocytes, isolated from the ovaries of slaughtered animals were assessed via the Brilliant Cresyl Blue test and directed to IVM. A number of oocytes were left to be analyzed as the 'before IVM' group. Oocyte mRNA was isolated and used for microarray analysis, which was subsequently validated via RT‑qPCR. The current study particularly focused on genes belonging to 'positive regulation of transcription, DNA‑dependent', 'positive regulation of gene expression', 'positive regulation of macromolecule metabolic process' and 'positive regulation of transcription from RNA polymerase II promoter' ontologies. FOS, VEGFA, ESR1, AR, CCND2, EGR2, ENDRA, GJA1, INHBA, IHH, INSR, APP, WWTR1, SMARCA1, NFAT5, SMAD4, MAP3K1, EGR1, RORA, ECE1, NR5A1, KIT, IKZF2, MEF2C, SH3D19, MITF and PSMB4 were all determined to be significantly altered (fold change, >|2|; P<0.05) among these groups, with their downregulation being observed after IVM. Genes with the most altered expressions were analyzed and considered to be potential markers of maturation associated with transcription regulation and macromolecule metabolism process.
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