ArticleMolecular medicine reports2020
miR‑21 promotes osteoclastogenesis through activation of PI3K/Akt signaling by targeting Pten in RAW264.7 cells.
Article in Molecular medicine reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- The characteristic expression of circulatingFrontiers in endocrinology · 2024Pooled it
- From force to resorption: mechanotransduction, osteoclasts, prevention.Progress in orthodontics · 2026Review
- Role of Cytokines in Bone Diseases and Their Therapeutic Application.ImmunoTargets and therapy · 2026Review
- MicroRNA, MicroRNA-lncRNA and MicroRNA-Circular RNA axes, and exosomal MicroRNAs: driving exercise-induced cardioprotection in heart failure.Frontiers in cell and developmental biology · 2026Review
- Exosomes in aging and age-related disorders: mechanisms, therapeutic potentials, and challenges.Journal of translational medicine · 2025Review
- Review
- LINC00482 targets miR-877-3p to modulate osteogenic differentiation in fracture patients.Journal of orthopaedic surgery and research · 2025Article
- Epigenetic Modulation and Bone Metastasis: Evolving Therapeutic Strategies.Pharmaceuticals (Basel, Switzerland) · 2025Review
- MiRNA-mRNA network in osteoporotic fractures proposes the functional mechanism of hsa-miR-32-3p/TNFSF11 axis.Journal of orthopaedic surgery and research · 2025Article
- microRNAs in Type 1 Diabetes: Roles, Pathological Mechanisms, and Therapeutic Potential.International journal of molecular sciences · 2025Review
- miR-374-5p inhibits osteogenesis by targeting PTEN/PI3K/AKT signaling pathway.Journal of orthopaedic surgery and research · 2025Article
- Development of biomaterials for bone tissue engineering based on bile acids.Journal of materials science. Materials in medicine · 2025Review
- Cardioepigenetics in action: aerobic exercise-induced modulation of miRNAs, lncRNAs, and chromatin remodeling in cardiovascular disease.Frontiers in cardiovascular medicine · 2025Review
- Research progress in periodontally accelerated osteogenic orthodontics.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2024Review
- Molecular Signaling Pathways and MicroRNAs in Bone Remodeling: A Narrative Review.Diseases (Basel, Switzerland) · 2024Review
- Role of extracellular vesicles associated with microRNAs and their interplay with cuproptosis in osteoporosis.Non-coding RNA research · 2024Review
- Effect of primary osteoblast-derived extracellular vesicles on osteoclast differentiation.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2024Article
- Review
- Subchondral osteoclasts and osteoarthritis: new insights and potential therapeutic avenues.Acta biochimica et biophysica Sinica · 2024Review
- MiR-29a-3p mediates phosphatase and tensin homolog and inhibits osteoarthritis progression.Functional & integrative genomics · 2024Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study aimed to investigate the effects of microRNA (miR)‑21 on osteoclastogenesis and its underlying molecular mechanisms. The expression levels of tartrate‑resistant acid phosphatase (TRAP) and miR‑21 were detected during osteoclastogenesis in receptor activator of NF‑κB ligand (RANKL)‑induced RAW264.7 cells via reverse transcription‑quantitative PCR. Bioinformatics and dual luciferase reporter assays were performed to analyze the association between miR‑21 and Pten. RANKL‑induced RAW264.7 cells were divided into the following groups: MiR‑negative control (NC), miR‑21 mimic, miR‑21 inhibitor and miR‑21 mimic + LY294002. The effects of miR‑21 on osteoclastogenesis and bone resorption were then detected using TRAP staining and a bone resorption assay. Pten, phosphorylated‑Akt and nuclear factor of activated T cell (NFATc1) expression levels were measured by western blotting to analyze the effects of miR‑21 on the PI3K/Akt signaling pathway. The present study revealed that miR‑21 was upregulated during osteoclastogenesis in RANKL‑induced RAW264.7 cells. Furthermore, miR‑21 negatively regulated Pten. Compared with the miR‑negative control (NC) group, the number of osteoclasts and the percentage of bone resorption were increased in the miR‑21 mimic group, whereas they were decreased in the miR‑21 inhibitor group. The number of osteoclasts and the percentage of bone resorption in the miR‑21 mimic + LY294002 group were lower than in the miR‑21 mimic group. Compared with the miR‑NC group, the protein expression levels of Pten were decreased, whereas p‑Akt and NFATc1 were increased in the miR‑21 mimic group. Conversely, Pten protein expression was increased, whereas p‑Akt and NFATc1 were decreased in the miR‑21 inhibitor group. In the miR‑21 mimic + LY294002 group, Pten protein expression was higher, and p‑Akt and NFATc1 were lower than in the miR‑21 mimic group. In conclusion, miR‑21 is upregulated during osteoclastogenesis, and may promote osteoclastogenesis and bone resorption through activating the PI3K/Akt signaling pathway via targeting Pten.
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