ArticleThe Journal of antibiotics2020
Discovery of "heat shock metabolites" produced by thermotolerant actinomycetes in high-temperature culture.
Article in The Journal of antibiotics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 30 citations in OpenAlex.
- Physicochemical and Functional Characterization of a Novel Extremophilic Exopolysaccharide Produced byMicroorganisms · 2026Article
- Unlocking the Bioactive Potential of Streptomyces: A Multidisciplinary Approach to Strain Improvement.MicrobiologyOpen · 2026Review
- Deciphering the multifarious biomedicinal properties of marine-derived Streptomyces kunmingensis through In vitro, In silico and GC-MS-based metabolomic approaches.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Extracellular Polymeric Substances Produced by Actinomycetes of the GenusInternational journal of molecular sciences · 2026Review
- The Heat-Shock Metabolite Streptolactam D, Produced by High-Temperature Culture ofJournal of the American Chemical Society · 2025Article
- Isolation, Structure, and Biological Activities of Exopolysaccharides from Actinomycetes: A Review.Current microbiology · 2024Review
- Activation of Secondary Metabolism and Protease Activity Mechanisms in the Black Koji MoldACS omega · 2024Article
- Integrative Genomics and Bioactivity-Guided Isolation of Novel Antimicrobial Compounds fromMolecules (Basel, Switzerland) · 2024Article
- A new analog of dihydroxybenzoic acid fromBeilstein journal of organic chemistry · 2024Article
- Methodology for awakening the potential secondary metabolic capacity in actinomycetes.Beilstein journal of organic chemistry · 2024Review
- Isolation, characterization and screening of actinomycetes associated with fijian ant-plant symbioses.Microbiology (Reading, England) · 2023Article
- Heat stress enhanced perylenequinones biosynthesis of Shiraia sp. Slf14(w) through nitric oxide formation.Applied microbiology and biotechnology · 2023Article
- RK-270D and E, Oxindole Derivatives fromJournal of microbiology and biotechnology · 2022Article
- Himalaquinones A-G, Angucyclinone-Derived Metabolites Produced by the Himalayan IsolateJournal of natural products · 2021Article
- Marine Actinomycetes, New Sources of Biotechnological Products.Marine drugs · 2021Review
- Discovery of novel secondary metabolites encoded in actinomycete genomes through coculture.Journal of industrial microbiology & biotechnology · 2021Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In actinomycetes, many secondary metabolite biosynthetic genes are not expressed under typical laboratory culture conditions and various efforts have been made to activate these dormant genes. In this study, we focused on high-temperature culture. First, we examined the thermotolerance of 3160 actinomycete strains from our laboratory culture collection and selected 57 thermotolerant actinomycetes that grew well at 45 °C. These 57 thermotolerant actinomycetes were cultured for 5 days in liquid medium at both 30 °C and 45 °C. Culture broths were extracted with 1-butanol, and each extract was subjected to LC/MS analysis. The metabolic profiles of each strain were compared between the 30 °C and 45 °C cultures. We found that almost half of these thermotolerant actinomycetes produced secondary metabolites that were detected only in the 45 °C culture. This result suggests that high-temperature culture induces the production of dormant secondary metabolites. These compounds were named "heat shock metabolites (HSMs)." To examine HSM production in more detail, 18 strains were selected at random from the initial 57 strains and cultivated in six different media at 30 °C and 45 °C; as before, metabolic profiles of each strain in each medium were compared between the 30 °C and 45 °C cultures. From this analysis, we found a total of 131 HSMs. We identified several angucycline-related compounds as HSMs from two thermotolerant Streptomyces species. Furthermore, we discovered a new compound, murecholamide, as an HSM from thermotolerant Streptomyces sp. AY2. We propose that high-temperature culture of actinomycetes is a convenient method for activating dormant secondary metabolite biosynthetic genes.
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