ArticleScientific reports2020
Immobilization of β-Glucosidase from Thermatoga maritima on Chitin-functionalized Magnetic Nanoparticle via a Novel Thermostable Chitin-binding Domain.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
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- A dual-functional dendrimer-based system integrating recombinant endolysin and cinnamic acid for enhanced endolysin stability and antibacterial activity against Acinetobacter baumannii.World journal of microbiology & biotechnology · 2026Article
- Green synthesis of magnetic bio-Graphene nanohybrid for the immobilization of hydrolytic enzymes towards sustainable bioconversion of cellulose.RSC advances · 2025Article
- Insight into the eminent biotechnological applications of xylanolytic enzymes for sustainable bioprocessing.3 Biotech · 2025Review
- An Overview of Microorganisms Immobilized in a Gel Structure for the Production of Precursors, Antibiotics, and Valuable Products.Gels (Basel, Switzerland) · 2024Review
- Advancements in enzyme immobilization on magnetic nanomaterials: toward sustainable industrial applications.RSC advances · 2024Review
- Biocatalytic Performance ofMicromachines · 2024Article
- Ultrasound-Promoted preparation and application of novel bifunctional core/shell FeScientific reports · 2023Article
- Microbial β-glucanases: production, properties, and engineering.World journal of microbiology & biotechnology · 2023Review
- Development of a Multi-Enzymatic Biocatalytic System through Immobilization on High Quality Few-Layer bio-Graphene.Nanomaterials (Basel, Switzerland) · 2022Article
- Improvement in the Thermostability of a Recombinant β-Glucosidase Immobilized in Zeolite under Different Conditions.Molecules (Basel, Switzerland) · 2022Article
- Evaluation of MScientific reports · 2022Article
- Surface-coated magnetic nanostructured materials for robust bio-catalysis and biomedical applications-A review.Journal of advanced research · 2022Review
- Mechano-chemical and biological energetics of immobilized enzymes onto functionalized polymers and their applications.Bioengineered · 2022Review
- Effective utilization of magnetic nano-coupled cloned β-xylanase in saccharification process.RSC advances · 2022Article
- Thiolation of Myco-Synthesized FeJournal of fungi (Basel, Switzerland) · 2022Article
- The Stability Improvement ofBiochemistry research international · 2022Article
- Production and immobilization of β-glucanase from Aspergillus niger with its applications in bioethanol production and biocontrol of phytopathogenic fungi.Scientific reports · 2021Article
- Recent Advances in Biopolymeric Membranes towards the Removal of Emerging Organic Pollutants from Water.Membranes · 2021Review
- Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives.Sensors (Basel, Switzerland) · 2021Review
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Authors and funding
6 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzyme immobilization is a powerful tool not only as a protective agent against harsh reaction conditions but also for the enhancement of enzyme activity, stability, reusability, and for the improvement of enzyme properties as well. Herein, immobilization of β-glucosidase from Thermotoga maritima (Tm-β-Glu) on magnetic nanoparticles (MNPs) functionalized with chitin (Ch) was investigated. This technology showed a novel thermostable chitin-binding domain (Tt-ChBD), which is more desirable in a wide range of large-scale applications. This exclusive approach was fabricated to improve the Galacto-oligosaccharide (GOS) production from a cheap and abundant by-product such as lactose through a novel green synthesis route. Additionally, SDS-PAGE, enzyme activity kinetics, transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FT-IR) revealed that among the immobilization strategies for Thermotoga maritime-β-Glucosidase thermostable chitin-binding domain (Tm-β-Glu-Tt-ChBD) on the attractive substrate; Ch-MNPs had the highest enzyme binding capacity and GOS production ratio when compared to the native enzyme. More interestingly, a magnetic separation technique was successfully employed in recycling the immobilized Tm-β-Glu for repetitive batch-wise GOS without significant loss or reduction of enzyme activity. This immobilization system displayed an operative stability status under various parameters, for instance, temperature, pH, thermal conditions, storage stabilities, and enzyme kinetics when compared with the native enzyme. Conclusively, the GOS yield and residual activity of the immobilized enzyme after the 10
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