Trial reportScientific reports2020
Diagnostic information Profiling and Evaluation of Causative Fungi of Fungal Keratitis Using High-throughput Internal Transcribed Spacer Sequencing.
Trial report in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- A Rare Case ofJournal of fungi (Basel, Switzerland) · 2025Article
- Pre-Colonization of Bacillus siamensis on Ocular Surface Mitigates Fusarium keratitis Through Direct Antifungal Activity and Pre-Activation of NF-κB Pathway.Investigative ophthalmology & visual science · 2025Article
- The Ocular Surface Microbiome in Homeostasis and Dysbiosis.Microorganisms · 2025Review
- Microbiologic analysis of 140 patients undergoing revision total knee arthroplasty due to periprosthetic joint infection and aseptic loosening: droplet digital PCR analysis.Journal of orthopaedic surgery and research · 2025Article
- Alterations in ocular fungal microbiota in patients with fungal keratitis: a comparative study from coastal regions of Eastern China.BMC ophthalmology · 2025Article
- The application of high-throughput sequencing technology in corneal diseases.International ophthalmology · 2024Review
- A Comprehensive Review of Microbial Biofilms on Contact Lenses: Challenges and Solutions.Infection and drug resistance · 2024Review
- Identification of Microbial Community in Otomycosis by Metagenomic Next Generation Sequencing (mNGS): Potential Implication of Treatment with Terbinafine.Mycopathologia · 2023Article
- Modern Technologies in Diagnosis of Fungal Keratitis (Review).Sovremennye tekhnologii v meditsine · 2023Review
- METTL3 Attenuates Inflammation in Fusarium solani-Induced Keratitis via the PI3K/AKT Signaling Pathway.Investigative ophthalmology & visual science · 2022Article
- Diagnostic methods for the etiological assessment of infectious corneal pathology (Review).Experimental and therapeutic medicine · 2022Review
- Identification of the Fungal Community in Otomycosis by Internal Transcribed Spacer Sequencing.Frontiers in microbiology · 2022Article
- Profiling of the Conjunctival Bacterial Microbiota Reveals the Feasibility of Utilizing a Microbiome-Based Machine Learning Model to Differentially Diagnose Microbial Keratitis and the Core Components of the Conjunctival Bacterial Interaction Network.Frontiers in cellular and infection microbiology · 2022Article
- Diagnostic armamentarium of infectious keratitis: A comprehensive review.The ocular surface · 2022Review
- Rapid Genomic Diagnosis of Fungal Infections in the Age of Next-Generation Sequencing.Journal of fungi (Basel, Switzerland) · 2021Review
- Profiling of Diagnostic Information of and Latent Susceptibility to Bacterial Keratitis From the Perspective of Ocular Bacterial Microbiota.Frontiers in cellular and infection microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Early and accurate diagnosis is essential for the targeted management of fungal keratitis (FK), which is one of the major blinding eye diseases worldwide. To elucidate the diagnostic information of high-throughput internal transcribed spacer (ITS) sequencing for identifying causative fungi of FK, 38 patients who were highly suspected of having FK were included in this research. In vivo confocal microscopy, potassium hydroxide smear, and fungal culture were performed to diagnose FK. Culture and ITS sequencing were used to identify causative fungi. We hypothesized that the dominant genus was the result of pathogen identification by ITS sequencing. Thirty-five patients were eventually diagnosed with FK, with fungal pathogens found by confocal microscopy in 27 patients (77.14%), by smear examination in 27 patients (77.14%), by culture in 25 patients (71.43%), and by ITS sequencing in 26 patients (74.29%). Eight causative fungal genera were determined by ITS sequencing, while five causative fungal genera were identified based on the morphology of the cultured pathogens. The results of ITS sequencing and culture were coincident in 10 patients with FK (28.57%). It is concluded that ITS sequencing, to some extent, challenged fungal culture and might be an optional complement in identifying fungal pathogens in corneas.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.