Evidence map›Paper›PMID 32014998›Full record

ArticleThe Journal of biological chemistry2020

A thermophilic phage uses a small terminase protein with a fixed helix-turn-helix geometry.

Janelle A Hayes, Brendan J Hilbert, Christl Gaubitz, Nicholas P Stone, Brian A Kelch

Open access · hybridAbstract read
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Article
  3. Structural basis for DNA recognition by a viral genome-packaging machine.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  4. The Expanding Diversity of Viruses from Extreme Environments.International journal of molecular sciences · 2024
    Review
  5. Viral Genomic DNA Packaging Machinery.Sub-cellular biochemistry · 2024
    Review
  6. Article
  7. Terminase Subunits from the Pseudomonas-Phage E217.Journal of molecular biology · 2022
    Article
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Janelle A HayesDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
Brendan J HilbertDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
Christl GaubitzDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
Nicholas P StoneDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655.
Brian A KelchDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655 brian.kelch@umassmed.edu.ORCID 0000-0002-1369-6989
University of Massachusetts Chan Medical School · US

Funding

The Mechanism and Regulation of ATP Hydrolysis in a Viral Genome Packaging MotorF31GM121019 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI HAYES, JANELLE · 2017 to 2018
$62k
NIGMS NIH HHS F31 GM121019
6 · The paper itself

Abstract

Tailed bacteriophages use a DNA-packaging motor to encapsulate their genome during viral particle assembly. The small terminase (TerS) component of this DNA-packaging machinery acts as a molecular matchmaker that recognizes both the viral genome and the main motor component, the large terminase (TerL). However, how TerS binds DNA and the TerL protein remains unclear. Here we identified gp83 of the thermophilic bacteriophage P74-26 as the TerS protein. We found that TerS

Indexed as

Adenosine TriphosphatasesBacteriophagesCryoelectron MicroscopyDNA, ViralEndodeoxyribonucleasesMolecular Dynamics SimulationMutagenesisProtein BindingProtein Conformation, alpha-HelicalProtein Structure, QuaternaryProtein SubunitsRecombinant ProteinsStatic ElectricityVirus AssemblyAdenosine Triphosphatasesbacteriophage SPP1 terminaseDNA, ViralEndodeoxyribonucleasesProtein SubunitsRecombinant Proteinsbacteriophagecryo-EMDNA-binding proteinDNA packagingDNA recognitionhelix–turn–helix domainmolecular motorsmall terminasethermophileviral motor

Identifiers

PMID32014998
PMCPMC7086035
OpenAlexW3004665771

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.