Evidence map›Paper›PMID 32014357›Full record

ArticleJournal of shoulder and elbow surgery2020

Novel in vivo mouse model of shoulder implant infection.

William L Sheppard, Gina M Mosich, Ryan A Smith, Christopher D Hamad, Howard Y Park, Stephen D Zoller, Rishi Trikha, Tatiana K McCoy, Rachel Borthwell, John Hoang and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of shoulder and elbow surgery, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

William L SheppardDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA.
Gina M MosichDepartment of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA.
Ryan A SmithDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Christopher D HamadDepartment of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA.
Howard Y ParkDepartment of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA.
Stephen D ZollerDepartment of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA.
Rishi TrikhaDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA.
Tatiana K McCoyDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Rachel BorthwellDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
John HoangDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Nicole TruongDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Nicolas CevallosDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Samuel ClarksonDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Kellyn R HoriDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Jan Maarten van DijlDepartment of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, Netherlands.
Kevin P FrancisDepartment of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA; PerkinElmer, Hopkinton, MA, USA.
Frank A PetriglianoDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA.
Nicholas M BernthalDavid Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA; Department of Orthopedic Surgery, University of California, Los Angeles, Santa Monica, CA, USA. Electronic address: nbernthal@mednet.ucla.edu.

Funding

Regenerative Musculoskeletal Medicine Training ProgramT32AR059033 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI John S Adams, Nicholas M. Bernthal · 2011 to 2026
$4.8M
Modifying and Delivering Antimicrobials to Prevent Orthopaedic Implant InfectionsK08AR069112 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BERNTHAL, NICHOLAS M. · 2016 to 2019
$704k
NIAMS NIH HHS K08 AR069112NIAMS NIH HHS T32 AR059033
6 · The paper itself

Abstract

backgroundAnimal models are used to guide management of periprosthetic implant infections. No adequate model exists for periprosthetic shoulder infections, and clinicians thus have no preclinical tools to assess potential therapeutics. We hypothesize that it is possible to establish a mouse model of shoulder implant infection (SII) that allows noninvasive, longitudinal tracking of biofilm and host response through in vivo optical imaging. The model may then be employed to validate a targeting probe (1D9-680) with clinical translation potential for diagnosing infection and image-guided débridement.

methodsA surgical implant was press-fit into the proximal humerus of c57BL/6J mice and inoculated with 2 μL of 1 × 10

resultsBoth inoculated groups established chronic infections. CFUs on postoperative day (POD) 42 were increased in the infected groups compared with the sterile group (P < .001). By POD 14, osteolysis was visualized in both infected groups. The e4 group developed catastrophic bone destruction by POD 42. The e3 group maintained a congruent shoulder joint. Targeting probes helped to visualize low-grade infections via fluorescence. DISCUSSION: Given bone destruction in the e4 group, a longitudinal, noninvasive mouse model of SII and chronic osteolysis was produced using e3 of S aureus Xen-36, mimicking clinical presentations of chronic SII.

conclusionThe development of this model provides a foundation to study new therapeutics, interventions, and host modifications.

Indexed as

Shoulder JointAnimalsBiofilmsDebridementDisease Models, AnimalFemaleMiceMice, Inbred C57BLPostoperative ComplicationsProsthesis-Related InfectionsShoulder ProsthesisStaphylococcal InfectionsStaphylococcus aureusarthroplastyimplantinfectionosteolysisosteomyelitisShoulder

Identifiers

PMID32014357
PMCPMC11037115

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.