ArticleMicrobial cell factories2020
Redesign and reconstruction of a steviol-biosynthetic pathway for enhanced production of steviol in Escherichia coli.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Article
- Metabolic engineering strategies: utilizing different microbial strains and advanced technologies for the synthesis of high-valued terpenoids.Antonie van Leeuwenhoek · 2026Review
- A Comprehensive Review on Steviol Glycosides: Sources, Properties, Bioactivities, Sensory-Functional Enhancement and Bioproduction Strategies.Plants (Basel, Switzerland) · 2026Review
- Advanced applications of synthetic biology technology in biosynthesis of bioactive compounds from medicinal plants.Chinese herbal medicines · 2026Review
- Water-soluble carotenoid: focused on natural carotenoid crocin.Food science and biotechnology · 2025Review
- Review
- CRISPR-Cas9-based genome-editing technologies in engineering bacteria for the production of plant-derived terpenoids.Engineering microbiology · 2024Review
- Advances in the Structures, Pharmacological Activities, and Biosynthesis of Plant Diterpenoids.Journal of microbiology and biotechnology · 2024Review
- Engineering Electron Transfer Pathway of Cytochrome P450s.Molecules (Basel, Switzerland) · 2024Review
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli.Microbial cell factories · 2024Article
- Sustainable biosynthesis of valuable diterpenes in microbes.Engineering microbiology · 2023Review
- Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors.Waste and biomass valorization · 2023Article
- Seeds ofInternational journal of molecular sciences · 2023Article
- DNA Free CRISPR/DCAS9 Based Transcriptional Activation System forPlants (Basel, Switzerland) · 2022Article
- De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts.Nature communications · 2022Article
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Bioproduction of Linalool From Paper Mill Waste.Frontiers in bioengineering and biotechnology · 2022Article
- Biotechnological interventions of in vitro propagation and production of valuable secondary metabolites in Stevia rebaudiana.Applied microbiology and biotechnology · 2021Review
- Microbial Production of Bioactive Retinoic Acid Using Metabolically EngineeredMicroorganisms · 2021Article
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4 authors.
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Abstract
backgroundSteviol glycosides such as stevioside have attracted the attention of the food and beverage industry. Recently, efforts were made to produce these natural sweeteners in microorganisms using metabolic engineering. Nonetheless, the steviol titer is relatively low in metabolically engineered microorganisms, and therefore a steviol-biosynthetic pathway in heterologous microorganisms needs to be metabolically optimized. The purpose of this study was to redesign and reconstruct a steviol-biosynthetic pathway via synthetic-biology approaches in order to overproduce steviol in Escherichia coli.
resultsA genome-engineered E. coli strain, which coexpressed 5' untranslated region (UTR)-engineered geranylgeranyl diphosphate synthase, copalyl diphosphate synthase, and kaurene synthase, produced 623.6 ± 3.0 mg/L ent-kaurene in batch fermentation. Overexpression of 5'-UTR-engineered, N-terminally modified kaurene oxidase of Arabidopsis thaliana yielded 41.4 ± 5 mg/L ent-kaurenoic acid. Enhanced ent-kaurenoic acid production (50.7 ± 9.8 mg/L) was achieved by increasing the cellular NADPH/NADP
conclusions5'-UTR engineering, the fusion protein approach, and redox balancing improved the steviol titer in flask fermentation and bioreactor fermentation. The expression engineering of steviol-biosynthetic enzymes and the genome engineering described here can serve as the basis for producing terpenoids-including steviol glycosides and carotenoids-in microorganisms.
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