ArticleInternational journal of molecular sciences2020
Changes in the Surface Expression of Intercellular Adhesion Molecule 3, the Induction of Apoptosis, and the Inhibition of Cell-Cycle Progression of Human Multidrug-Resistant Jurkat/A4 Cells Exposed to a Random Positioning Machine.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 18 citations in OpenAlex.
- Microgravity and cancer cells: from molecular mechanisms to therapeutic strategies.Cell death discovery · 2026Review
- Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.International journal of molecular sciences · 2024Review
- Combined Biological and Numerical Modeling Approach for Better Understanding of the Cancer Viability and Apoptosis.Pharmaceutics · 2023Article
- Mechano-Immunomodulation in Space: Mechanisms Involving Microgravity-Induced Changes in T Cells.Life (Basel, Switzerland) · 2021Review
- Rac1/Wave2/Arp3 Pathway Mediates Rat Blood-Brain Barrier Dysfunction under Simulated Microgravity Based on Proteomics Strategy.International journal of molecular sciences · 2021Article
- Role of Apoptosis in Wound Healing and Apoptosis Alterations in Microgravity.Frontiers in bioengineering and biotechnology · 2021Review
- Influence of Microgravity on Apoptosis in Cells, Tissues, and Other Systems In Vivo and In Vitro.International journal of molecular sciences · 2020Review
- The Impact of Spaceflight and Simulated Microgravity on Cell Adhesion.International journal of molecular sciences · 2020Review
- Microgravity and Cell Adherence.International journal of molecular sciences · 2020Article
- Changes in the Surface Expression of Intercellular Adhesion Molecule 3, the Induction of Apoptosis, and the Inhibition of Cell-Cycle Progression of Human Multidrug-Resistant Jurkat/A4 Cells Exposed to a Random Positioning Machine.International journal of molecular sciences · 2020Article
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7 authors at 1 institution in 1 country.
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Abstract
Experiments from flight- and ground-based model systems suggest that unexpected alterations of the human lymphoblastoid cell line Jurkat, as well as effects on cell growth, metabolism, and apoptosis, can occur in altered gravity conditions. Using a desktop random positioning machine (RPM), we investigated the effects of simulated microgravity on Jurkat cells and their multidrug-resistant subline, Jurkat/A4 cells. The viability of Jurkat/A4 cells decreased after simulated microgravity in contrast with the Jurkat cells. At the same time, the viability between the experimental Jurkat cells and control Jurkat cells was not significantly different. Of note, Jurkat cells appeared as less susceptible to apoptosis than their multidrug-resistant clone Jurkat/A4 cells, whereas cell-cycle analysis showed that the percentage of Jurkat/A4 cells in the S-phase was increased after 72 and 96 h of RPM-simulated microgravity relative to their static counterparts. The differences in Jurkat cells at all phases between static and simulated microgravity were not significant. The surface expression of the intercellular adhesion molecule 3 (ICAM-3)-also known as cluster of differentiation (CD)50-protein was changed for Jurkat/A4 cells following exposure to the RPM. Changes in cell morphology were observed in the Jurkat/A4 cells after 96 h of RPM-simulated microgravity. Thus, we concluded that Jurkat/A4 cells are more sensitive to RPM-simulated microgravity as compared with the parental Jurkat cell line. We also suggest that intercellular adhesion molecule 3 may be an important adhesion molecule involved in the induction of leukocyte apoptosis. The Jurkat/A4 cells with an acquired multidrug resistance phenotype could be a useful model for studying the effects of simulated microgravity and testing anticancer drugs.
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