Evidence map›Paper›PMID 32012692›Full record

ArticleInternational journal of molecular sciences2020

Resveratrol as Inducer of Autophagy, Pro-Survival, and Anti-Inflammatory Stimuli in Cultured Human RPE Cells.

Natasha Josifovska, Réka Albert, Richárd Nagymihály, Lyubomyr Lytvynchuk, Morten C Moe, Kai Kaarniranta, Zoltán J Veréb, Goran Petrovski

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
5.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 57 citations in OpenAlex.

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  16. Secondary Metabolites fromMolecules (Basel, Switzerland) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 4 countries.

Natasha JosifovskaDepartment of Ophthalmology, Center for Eye Research, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway.
Réka AlbertDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, 6720 Szeged, Hungary.
Richárd NagymihályDepartment of Ophthalmology, Center for Eye Research, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway.
Lyubomyr LytvynchukDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, 6720 Szeged, Hungary.
Morten C MoeDepartment of Ophthalmology, Center for Eye Research, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway.
Kai KaarnirantaDepartment of Ophthalmology, Institute of Clinical Medicine, University of Eastern Finland and Kuopio University Hospital, 70210 Kuopio, Finland.
Zoltán J VerébDepartment of Ophthalmology, Faculty of Medicine, University of Szeged, 6720 Szeged, Hungary.ORCID 0000-0002-9518-2155
Goran PetrovskiDepartment of Ophthalmology, Center for Eye Research, Oslo University Hospital and University of Oslo, 0450 Oslo, Norway.
Oslo University Hospital · NOUniversity of Szeged · HUJustus-Liebig-Universität Gießen · DEUniversity of Eastern Finland · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

methodsCultured ARPE-19 cells were exposed to 10 and 50 μM resveratrol. Cell survival/death was determined by annexin-FITC/propidium iodide using flow cytometry, while autophagy was studied by detecting autophagic vacuoles formation (acridine orange and transmission electron microscopy), as well as LC3II/I ratio and p62 expression by Western blot. In addition, time-lapse confocal microscopy of a pDENDRA-LC3 expression vector was performed to detect autophagy in transfected ARPE-19 cells under the different treatment conditions. Inhibition of proteasomal and autophagy-lysosomal fusion was carried out by MG-132 and chloroquine, respectively, while induction of autophagy was achieved by rapamycin treatment. Detection of secreted cytokines by ARPE-19 cells using Human XL Cytokine Array was performed under oxidative stress (H

resultsResveratrol induced autophagy in ARPE-19 cells as determined by augmented presence of autophagic vacuoles, increased LC3II/I ratio and decreased p62 expression, as well as time-lapse confocal microscopy using pDENDRA-LC3 expression vector. Resveratrol acted similarly to proteasomal inhibition and downstream of mammalian target of rapamycin (mTOR), since upstream inhibition of autophagy by 3-methyladenine could not inhibit autophagy in ARPE-19 cells. Co-treatmeant by rapamycin and/or proteasome inhibition showed no additive effect upon autophagy induction. ARPE-19 cells treated by resveratrol showed lower cell death rate compared to untreated controls. Resveratrol induced a specific anti-inflammatory response in ARPE-19 cells.

conclusionsResveratrol can induce autophagy, pro-survival, and anti-inflammatory stimuli in ARPE-19 cells, properties which could be plausible to formulate future treatment modalities for AMD.

Indexed as

Anti-Inflammatory AgentsAutophagyCell DeathCell LineCells, CulturedCell SurvivalEpithelial CellsHumansProteasome Endopeptidase ComplexResveratrolRetinal Pigment EpitheliumAnti-Inflammatory AgentsProteasome Endopeptidase ComplexResveratrolage-related macular degenerationautophagyinflammationproteasomal inhibitionprotein arrayresveratrolretinal pigment epitheliumsurvival

Identifiers

PMID32012692
PMCPMC7036848
OpenAlexW3001067362

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.