ArticleMicrobial biotechnology2020
Production of rebaudioside D from stevioside using a UGTSL2 Asn358Phe mutant in a multi-enzyme system.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 48 citations in OpenAlex.
- Dual-Enzyme Biocatalytic Synthesis of Novel Rebaudioside M Analogs with Enhanced Aqueous Solubility, Physicochemical Stability, and Sensory Profiles.Foods (Basel, Switzerland) · 2026Article
- Discovery and engineering of UDP-glycosyltransferase and its applications in natural sweetener glycosylation.World journal of microbiology & biotechnology · 2026Review
- Structural Elucidation of Quinovose-Containing Steviol Glycosides from Enzymatic Biotransformation ofMolecules (Basel, Switzerland) · 2026Article
- A Comprehensive Review on Steviol Glycosides: Sources, Properties, Bioactivities, Sensory-Functional Enhancement and Bioproduction Strategies.Plants (Basel, Switzerland) · 2026Review
- Advances in semi-rational design of terpene synthases and their modifying enzymes.AMB Express · 2025Review
- Multi-strategy ugt mining, modification and glycosyl donor synthesis facilitate the production of triterpenoid saponins.Frontiers in plant science · 2025Review
- Recent Advances in the Biosynthesis of Natural Sugar Substitutes in Yeast.Journal of fungi (Basel, Switzerland) · 2023Review
- Engineering of cyclodextrin glycosyltransferase improves the conversion efficiency of rebaudioside A to glucosylated steviol glycosides and increases the content of short-chain glycosylated steviol glycoside.Microbial cell factories · 2023Article
- Cold Plasma-Induced Changes inPlants (Basel, Switzerland) · 2023Article
- Applications of protein engineering in the microbial synthesis of plant triterpenoids.Synthetic and systems biotechnology · 2023Review
- Identification of sucrose synthase fromFrontiers in microbiology · 2023Article
- Probiotic lactic acid bacteria alleviate pediatric IBD and remodel gut microbiota by modulating macrophage polarization and suppressing epithelial apoptosis.Frontiers in microbiology · 2023Article
- De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts.Nature communications · 2022Article
- The Potential of Cold Plasma and Electromagnetic Field as Stimulators of Natural Sweeteners Biosynthesis inPlants (Basel, Switzerland) · 2022Article
- Highly efficient production of rebaudioside D enabled by structure-guided engineering of bacterial glycosyltransferase YojK.Frontiers in bioengineering and biotechnology · 2022Article
- Improving Thermostability and Catalytic Activity of Glycosyltransferase FromFrontiers in bioengineering and biotechnology · 2022Article
- Metabolic engineering for the synthesis of steviol glycosides: current status and future prospects.Applied microbiology and biotechnology · 2021Review
- Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade.RSC advances · 2021Article
- β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts.Frontiers in bioengineering and biotechnology · 2021Article
- Production of rebaudioside D from stevioside using a UGTSL2 Asn358Phe mutant in a multi-enzyme system.Microbial biotechnology · 2020Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
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Abstract
Rebaudioside D is a sweetener from Stevia rebaudiana with superior sweetness and organoleptic properties, but its production is limited by its minute abundance in S. rebaudiana leaves. In this study, we established a multi-enzyme reaction system with S. rebaudiana UDP-glycosyltransferases UGT76G1, Solanum lycopersicum UGTSL2 and Solanum tuberosum sucrose synthase StSUS1, achieving a two-step glycosylation of stevioside to produce rebaudioside D. However, an increase in the accumulation of rebaudioside D required the optimization of UGTSL2 catalytic activity towards glucosylation of rebaudioside A and reducing the formation of the side-product rebaudioside M2. On the basis of homology modelling and structural analysis, Asn358 in UGTSL2 was subjected to saturating mutagenesis, and the Asn358Phe mutant was used instead of wild-type UGTSL2 for bioconversion. The established multi-enzyme reaction system employing the Asn358Phe mutant produced 14.4 g l
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