Evidence map›Paper›PMID 32010502›Full record

ArticleBiomedical optics express2020

Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings.

Wenbin Zhu, Giacomo Pirovano, Patrick K O'Neal, Cheng Gong, Nachiket Kulkarni, Christopher D Nguyen, Christian Brand, Thomas Reiner, Dongkyun Kang

Open access · goldAbstract read
In one paragraph

Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Improved radiosynthesis ofInternational journal of radiation biology · 2023
    Article
  6. Article
  7. A review on low-cost microscopes for Open Science.Microscopy research and technique · 2022
    Review
  8. Article
  9. Article
  10. PARP1: A Potential Molecular Marker to Identify Cancer During Colposcopy Procedures.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2021
    Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Wenbin ZhuCollege of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.ORCID https://orcid.org/0000-0002-8754-6529
Giacomo PirovanoDepartment of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Patrick K O'NealCollege of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.
Cheng GongCollege of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.ORCID https://orcid.org/0000-0002-0026-2006
Nachiket KulkarniCollege of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.
Christopher D NguyenCollege of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.
Christian BrandSummit Biomedical Imaging, LLC., New York, NY 10027, USA.
Thomas ReinerDepartment of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Dongkyun KangCollege of Optical Sciences, University of Arizona, Tucson, AZ 85721, USA.
University of Arizona · USMemorial Sloan Kettering Cancer Center · US

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Hongbiao Carl Lekaye · 1985 to 2026
$347.4M
Radioiodinated Multifunctional PARP1 Imaging Probes for Diagnosis and TherapyR01CA204441 · NCI · SLOAN-KETTERING INST CAN RESEARCH · PI PILLARSETTY, NAGAVARAKISHORE · 2016 to 2020
$2.9M
Rapid, quantitative and affordable point-of-care diagnostics for oral and oropharyngeal cancerR43CA228815 · NCI · SUMMIT BIOMEDICAL IMAGING, LLC · PI BRAND, CHRISTIAN, REINER, THOMAS · 2018 to 2018
$300k
NCI NIH HHS P30 CA008748NCI NIH HHS R01 CA204441NCI NIH HHS R43 CA228815
6 · The paper itself

Abstract

Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.

Identifiers

PMID32010502
PMCPMC6968742
OpenAlexW2993849036

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.