ArticleBiomedical optics express2020
How to define and optimize axial resolution in light-sheet microscopy: a simulation-based approach.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells.ACS photonics · 2026Article
- An optimized click chemistry method allows visualization of proliferating neuronal progenitors in the mouse brain.Cell reports methods · 2025Article
- Lattice light-sheet microscopy allows for super-resolution imaging of receptors in leaf tissue.Biophysical journal · 2025Article
- Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy.Biomedical optics express · 2024Article
- Model based optimization for refractive index mismatched light sheet imaging.Optics express · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples.Nature biomedical engineering · 2024Article
- User-friendly oblique plane microscopy on a fully functional commercially available microscope base.Biomedical optics express · 2024Article
- Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning.Intelligent computing (Washington, D.C.) · 2024Article
- Enhancing Light-Sheet Fluorescence Microscopy Illumination Beams through Deep Design Optimization.bioRxiv : the preprint server for biology · 2023Article
- Article
- Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy.Cell reports methods · 2023Article
- Characterization, comparison, and optimization of lattice light sheets.Science advances · 2023Article
- Isotropic imaging across spatial scales with axially swept light-sheet microscopy.Nature protocols · 2022Review
- A quantitative analysis of various patterns applied in lattice light sheet microscopy.Nature communications · 2022Article
- Online Monitoring of the Concentrations of Amorphous and Crystalline Mesoscopic Species Present in Solution.Crystal growth & design · 2022Article
- Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.International journal of molecular sciences · 2022Review
- Segmentation of Tissues and Proliferating Cells in Light-Sheet Microscopy Images of Mouse Embryos Using Convolutional Neural Networks.IEEE access : practical innovations, open solutions · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
"How thick is your light sheet?" is a question that has been asked frequently after talks showing impressive renderings of 3D data acquired by a light-sheet microscope. This question is motivated by the fact that most of the time the thickness of the light-sheet is uniquely associated to the axial resolution of the microscope. However, the link between light-sheet thickness and axial resolution has never been systematically assessed and it is still unclear how both are connected. The question is not trivial because commonly employed measures cannot readily be applied or do not lead to easily interpretable results for the many different types of light sheet. Here, we introduce a set of intuitive measures that helps to define the relationship between light sheet thickness and axial resolution by using simulation data. Unexpectedly, our analysis revealed a trade-off between better axial resolution and thinner light-sheet thickness. Our results are surprising because thicker light-sheets that provide lower image contrast have previously not been associated with better axial resolution. We conclude that classical Gaussian illumination beams should be used when image contrast is most important, and more advanced types of illumination represent a way to optimize axial resolution at the expense of image contrast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.