ArticleFEMS microbiology ecology2020
Total RNA sequencing reveals multilevel microbial community changes and functional responses to wood ash application in agricultural and forest soil.
Article in FEMS microbiology ecology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
- From dividing to dormant: embracing the full activity spectrum for environmental microorganisms.Microbiology and molecular biology reviews : MMBR · 2026Review
- Bacterial and archaeal communities reflect long-term pH shift induced by ash fertilization in boreal peatland forest soils.FEMS microbiology ecology · 2026Article
- Transcriptional and metabolic stasis define desiccation-induced dormancy in the soil bacteriummSystems · 2026Article
- Effects of Wood Biomass Combustion Residues on the Structure, Diversity, and Trophic Functions of Soil Fungi.International journal of molecular sciences · 2026Article
- Concurrent stimulation of diflufenican biodegradation and changes in the active microbiome in gravel revealed by Total RNA.Microbiology spectrum · 2025Article
- Arctic Soil C and N Cycling Are Linked With Microbial Adaptations During Drought.Global change biology · 2025Article
- Article
- Small sample amounts from rhizosphere of barley maintain microbial community structure and diversity revealed by total RNA sequencing.Plant methods · 2025Article
- Towards improved biofilm models.Nature reviews. Microbiology · 2025Review
- The link between antibiotic resistance level and soil physico-chemical properties.Frontiers in microbiology · 2025Article
- Evaluation of commercial RNA extraction kits for long-read metatranscriptomics in soil.Microbial genomics · 2024Article
- Total RNA analysis of the active microbiome on moving bed biofilm reactor carriers under incrementally increasing micropollutant concentrations.FEMS microbiology ecology · 2024Article
- Bio-based resources: systemic & circular solutions for (agro)environmental services.RSC advances · 2024Review
- Searching for Chemical Agents Suppressing Substrate Microbiota in White-Rot Fungi Large-Scale Cultivation.Microorganisms · 2024Article
- Metagenomics and metatranscriptomics as potential driving forces for the exploration of diversity and functions of micro-eukaryotes in soil.3 Biotech · 2023Review
- Abrupt permafrost thaw triggers activity of copiotrophs and microbiome predators.FEMS microbiology ecology · 2023Article
- Predicting environmental stressor levels with machine learning: a comparison between amplicon sequencing, metagenomics, and total RNA sequencing based on taxonomically assigned data.Frontiers in microbiology · 2023Article
- Impact of Soil Fertilized with Biomass Ash on Depth-Related Variability of Culturable Bacterial Diversity and Selected Physicochemical Parameters in Spring Barley Cultivation.International journal of environmental research and public health · 2022Article
- Forest soil biotic communities show few responses to wood ash applications at multiple sites across Canada.Scientific reports · 2022Article
- Influence of planting yellowhorn (PeerJ · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recycling of wood ash from energy production may counteract soil acidification and return essential nutrients to soils. However, wood ash amendment affects soil physicochemical parameters that control composition and functional expression of the soil microbial community. Here, we applied total RNA sequencing to simultaneously assess the impact of wood ash amendment on the active soil microbial communities and the expression of functional genes from all microbial taxa. Wood ash significantly affected the taxonomic (rRNA) as well as functional (mRNA) profiles of both agricultural and forest soil. Increase in pH, electrical conductivity, dissolved organic carbon and phosphate were the most important physicochemical drivers for the observed changes. Wood ash amendment increased the relative abundance of the copiotrophic groups Chitinonophagaceae (Bacteroidetes) and Rhizobiales (Alphaproteobacteria) and resulted in higher expression of genes involved in metabolism and cell growth. Finally, total RNA sequencing allowed us to show that some groups of bacterial feeding protozoa increased concomitantly to the enhanced bacterial growth, which shows their pivotal role in the regulation of bacterial abundance in soil.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.