Evidence map›Paper›PMID 32006110›Full record

ArticlePlanta2020

Insights obtained using different modules of the cotton uceA1.7 promoter.

Marcos Fernando Basso, Isabela Tristan Lourenço-Tessutti, Carlos Busanello, Clidia Eduarda Moreira Pinto, Elínea de Oliveira Freitas, Thuanne Pires Ribeiro, Janice de Almeida Engler, Antonio Costa de Oliveira, Carolina Vianna Morgante, Marcio Alves-Ferreira and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.6field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Marcos Fernando BassoEmbrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-901, Brazil. marcosbiotec@gmail.com.ORCID http://orcid.org/0000-0001-8192-8959
Isabela Tristan Lourenço-TessuttiEmbrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-901, Brazil.
Carlos BusanelloFederal University of Pelotas, Capão Do Leão, RS, 96160-000, Brazil.
Clidia Eduarda Moreira PintoEmbrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-901, Brazil.
Elínea de Oliveira FreitasEmbrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-901, Brazil.
Thuanne Pires RibeiroEmbrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-901, Brazil.
Janice de Almeida EnglerUMR Institut Sophia Agrobiotech INRA/CNRS/UNS, Sophia Antipolis, France.
Antonio Costa de OliveiraFederal University of Pelotas, Capão Do Leão, RS, 96160-000, Brazil.
Carolina Vianna MorganteEmbrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-901, Brazil.
Marcio Alves-FerreiraFederal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-901, Brazil.
Maria Fatima Grossi-de-SaEmbrapa Genetic Resources and Biotechnology, PqEB Final, W5 Norte, PO Box 02372, Brasília, DF, 70770-901, Brazil. fatima.grossi@embrapa.br.
Brazilian Agricultural Research Corporation · BRUniversidade de Brasília · BRUniversidade Federal de Pelotas · BRCentre National de la Recherche Scientifique · FRUniversidade Federal do Rio de Janeiro · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MAIN

conclusionThe structure of the cotton uceA1.7 promoter and its modules was analyzed; the potential of their key sequences has been confirmed in different tissues, proving to be a good candidate for the development of new biotechnological tools. Transcriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5'-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops.

Indexed as

5' Untranslated RegionsArabidopsisCaulimovirusGene Expression Regulation, PlantGenes, PlantGenes, ReporterGenetic EngineeringGossypiumPlants, Genetically ModifiedPromoter Regions, Genetic5' Untranslated RegionsCotton constitutive promoterGene expressionNew biotechnological toolsTranscriptional core promoterTransgenic crops

Identifiers

PMID32006110
OpenAlexW3003613961

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.