ArticlePlant biotechnology journal2020
Transactivation of Sus1 and Sus2 by Opaque2 is an essential supplement to sucrose synthase-mediated endosperm filling in maize.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 81 citations in OpenAlex.
- Common genetic control for grain filling duration and kernel weight in grain sorghum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- ZmSK1 encodes a sucrose synthase essential for starch synthesis and endosperm development in maize.Plant molecular biology · 2026Article
- The Plant Sucrose Synthase Gene Family: Multi-Level Regulatory Networks and Functional Diversification in Plants.Biomolecules · 2026Review
- Identification of transcription factors regulating starch biosynthesis in maize through integrated GWAS and transcriptomic analysis.BMC plant biology · 2026Article
- Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.Journal of experimental botany · 2026Article
- From development to yield: genetic and molecular regulation of agronomic traits in maize seeds.Frontiers in plant science · 2026Review
- Regulatory Mechanisms of Free Umami Amino Acid Accumulation in Fresh Waxy Kernels: Insights from Transcriptome and Metabolomics Analyses.Foods (Basel, Switzerland) · 2025Article
- The bZIP transcription factor RISBZ1 balances grain filling and ER stress response in rice grains.Plant communications · 2025Article
- Transcriptional regulation and functional research of sucrose synthase in plant development.Planta · 2025Review
- Genome-Wide Identification of Luffa Sucrose Synthase Genes RevealsInternational journal of molecular sciences · 2025Article
- Integrated metabolomic and transcriptomic analyses reveal that starch and sucrose metabolism regulate maize kernel hardness.Frontiers in plant science · 2025Article
- Natural variation of WBR7 confers rice high yield and quality by modulating sucrose supply in sink organs.Plant biotechnology journal · 2024Article
- Central Roles of ZmNAC128 and ZmNAC130 in Nutrient Uptake and Storage during Maize Grain Filling.Genes · 2024Article
- Linkage mapping and genomic prediction of grain quality traits in tropical maize (Frontiers in genetics · 2024Article
- NAKED ENDOSPERM1, NAKED ENDOSPERM2, and OPAQUE2 interact to regulate gene networks in maize endosperm development.The Plant cell · 2023Article
- The transcription factors ZmNAC128 and ZmNAC130 coordinate with Opaque2 to promote endosperm filling in maize.The Plant cell · 2023Article
- RNA polymerase common subunit ZmRPABC5b is transcriptionally activated by Opaque2 and essential for endosperm development in maize.Nucleic acids research · 2023Article
- Genome-Wide Identification and Expression Analysis of the Sucrose Synthase Gene Family in Sweet Potato and Its Two Diploid Relatives.International journal of molecular sciences · 2023Article
- Article
- The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit.Plant physiology · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The endosperm-specific transcription factor Opaque2 (O2) acts as a central regulator for endosperm filling, but its functions have not been fully defined. Regular o2 mutants exhibit a non-vitreous phenotype, so we used its vitreous variety Quality Protein Maize to create EMS-mutagenesis mutants for screening o2 enhancers (oen). A mutant (oen1) restored non-vitreousness and produced a large cavity in the seed due to severely depleted endosperm filling. When oen1 was introgressed into inbred W64A with a normal O2 gene, the seeds appeared vitreous but had a shrunken crown. oen1 was determined to encode Shrunken1 (Sh1), a sucrose synthase (SUS, EC 2.4.1.13). Maize contains three SUS-encoding genes (Sh1, Sus1, and Sus2) with Sh1 contributing predominantly to the endosperm. We determined SUS activity and found a major and minor reduction in oen1 and o2, respectively. In o2;oen1-1, SUS activity was further decreased. We found all Sus gene promoters contain at least one O2 binding element that can be specifically recognized and be transactivated by O2. Sus1 and Sus2 promoters had a much stronger O2 transactivation than Sh1, consistent with their transcript reduction in o2 endosperm. Although sus1 and sus2 alone or in combination had no perceptible phenotype, either of them could dramatically enhance seed opacity and cavity in sh1, indicating that transactivation of Sus1 and Sus2 by O2 supplements SUS-mediated endosperm filling in maize. Our findings demonstrate that O2 transcriptionally regulates the metabolic source entry for protein and starch synthesis during endosperm filling.
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