ArticlePloS one2020
High prevalence of Phasi Charoen-like virus from wild-caught Aedes aegypti in Grenada, W.I. as revealed by metagenomic analysis.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- The application of metagenomics in the detection of arboviruses in mosquitoes (Diptera: Culicidade): a systematic review.Revista do Instituto de Medicina Tropical de Sao Paulo · 2025Pooled it
- Spatiotemporal variation in the microbiome ofMicrobiology spectrum · 2026Article
- Virome profiling of Culex tarsalis through small RNA-seq: A challenge of suboptimal samples.PLoS neglected tropical diseases · 2025Article
- Virome sequencing and analysis of Aedes aegypti and Aedes albopictus from ecologically different sites in the Philippines.Parasites & vectors · 2025Article
- Metagenomic analysis of mosquitoes from Kangerlussuaq, Greenland reveals a unique virome.Scientific reports · 2025Article
- An Exploration of the Viral Coverage of Mosquito Viromes Using Meta-Viromic Sequencing: A Systematic Review and Meta-Analysis.Microorganisms · 2024Review
- Influence of dengue virus serotypes on the abundance ofJournal of virology · 2024Article
- Larvicidal and anti-termite activities of microbial biosurfactant produced by Enterobacter cloacae SJ2 isolated from marine sponge Clathria sp.Scientific reports · 2023Article
- Longitudinal Study of Viral Diversity Associated with Mosquito Species Circulating in Cambodia.Viruses · 2023Article
- Leveraging insect-specific viruses to elucidate mosquito population structure and dynamics.PLoS pathogens · 2023Review
- Composition and global distribution of the mosquito virome - A comprehensive database of insect-specific viruses.One health (Amsterdam, Netherlands) · 2023Article
- Role of the Microbiome inViruses · 2023Review
- Aedes aegypti and Ae. albopictus microbiome/virome: new strategies for controlling arboviral transmission?Parasites & vectors · 2022Review
- Swapping Metagenomics Preprocessing Pipeline Components Offers Speed and Sensitivity Increases.mSystems · 2022Article
- Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India.PloS one · 2022Article
- Local-scale virome depiction in Medellín, Colombia, supports significant differences between Aedes aegypti and Aedes albopictus.PloS one · 2022Article
- Characterization of the virome associated with Haemagogus mosquitoes in Trinidad, West Indies.Scientific reports · 2021Article
- The Kidney-Associated Microbiome of Wild-CaughtAnimals : an open access journal from MDPI · 2021Article
- Detection of Cell-Fusing Agent virus across ecologically diverse populations ofWellcome open research · 2020Article
- Metagenomic analysis of Aedes aegypti and Culex quinquefasciatus mosquitoes from Grenada, West Indies.PloS one · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arboviruses cause diseases of significant global health concerns. Interactions between mosquitoes and their microbiota as well as the important role of this interaction in the mosquito's capacity to harbor and transmit pathogens have emerged as important fields of research. Aedes aegypti is one of the most abundant mosquitoes in many geographic locations, a vector capable of transmitting a number of arboviruses such as dengue and Zika. Currently, there are few studies on the metavirome of this mosquito particularly in the Americas. This study analyzes the metavirome of A. aegypti from Grenada, a Caribbean nation with tropical weather, abundant A. aegypti, and both endemic and arboviral pathogens transmitted by this mosquito. Between January and December 2018, 1152 mosquitoes were collected from six semi-rural locations near houses in St. George Parish, Grenada, by weekly trapping using BG-Sentinel traps. From these, 300 A. aegypti were selected for analysis. The metavirome was analyzed using the Illumina HiSeq 1500 for deep sequencing. The generation sequencing library construction protocol used was NuGEN Universal RNA with an average read length of 125 bp. Reads were mapped to the A. aegypti assembly. Non-mosquito reads were analyzed using the tools FastViromeExplorer. The NCBI total virus, RNA virus, and eukaryotic virus databases were used as references. The metagenomic comparison analysis showed that the most abundant virus-related reads among all databases and assemblies was Phasi Charoen-like virus. The Phasi Charoen-like virus results are in agreement to other studies in America, Asia and Australia. Further studies using wild-caught mosquitoes is needed to assess the impact of this insect-specific virus on the A. aegypti lifecycle and vector capacity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.