ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2020
Development and application of oligonucleotide-based chromosome painting for chromosome 4D of Triticum aestivum L.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Chromosome painting in plants: history and future perspectives.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Review
- Development and characterization of a novel compensating wheat-Dasypyrum villosum interstitial translocation line Dv6-IT1 carrying the powdery mildew resistance gene PmV.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Cytogenomic and phylogenomic evidence for new infrageneric relationships in Macroptilium (Benth.) beans.Annals of botany · 2026Article
- Whole Genome Development of Specific Alien-Chromosome Oligo (SAO) Markers for Wild Peanut Chromosomes Based on Chorus2.Plants (Basel, Switzerland) · 2025Article
- Comprehensive mapping of molecular cytogenetic markers in pitaya (Frontiers in plant science · 2024Article
- Chromosome-specific painting in Thinopyrum species using bulked oligonucleotides.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Oligonucleotide Fluorescence In Situ Hybridization: An Efficient Chromosome Painting Method in Plants.Plants (Basel, Switzerland) · 2023Review
- Transferring a new Fusarium head blight resistance locus FhbRc1 from Roegneria ciliaris into wheat by developing alien translocation lines.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Reconstruction of karyotypic evolution in Saccharum spontaneum species by comparative oligo-FISH mapping.BMC plant biology · 2022Article
- Accurate Chromosome Identification in the Prunus SubgenusInternational journal of molecular sciences · 2022Article
- Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes.BMC genomics · 2022Article
- Advances in the Molecular Cytogenetics of Bananas, Family Musaceae.Plants (Basel, Switzerland) · 2022Review
- Oligo-FISH barcode in beans: a new chromosome identification system.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- BAC- and oligo-FISH mapping reveals chromosome evolution among Vigna angularis, V. unguiculata, and Phaseolus vulgaris.Chromosoma · 2021Article
- Chromosomal Characterization ofInternational journal of molecular sciences · 2021Article
- Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds.Chromosoma · 2021Article
- A universal chromosome identification system for maize and wild Zea species.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
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12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome painting is a useful technique for distinguishing specific chromosomes (fragments), elucidating the genetic relationships of different genomes or chromosomes, and identifying chromosomal rearrangements. The development of chromosome- or genome-specific probes is fundamental for chromosome painting. The possibility for developing such probes specifically painting homoeologous chromosomes in allopolyploid species has been questioned since that chromosomes belonging to the same homoeologous group share highly conserved sequences. In the present study, we attempted to construct a wheat chromosome 4D-specific oligo probe library by selecting 4D-specific sequences in reference genome of common wheat cv. Chinese Spring (CS, 2n = 6x = 42, AABBDD). The synthesized library contains 27,392 oligos. Oligo painting using the probe library confirmed its specificity, shown by that only chromosome 4D could be painted in three wheat genotypes and CS nulli-tetrasomic line N4AT4D. Oligo painting was successfully used to define the 4D breakpoints in CS deletion lines involving 4D and two wheat-Haynaldia villosa 4D-4V translocation lines. Thirteen wheat relatives and a Triticum durum-H. villosa amphiploid were used for oligo painting. Except the 4D in two Aegilops tauschii accessions, the 4M in Ae. comosa and 4U in Ae. umbellulata could be painted. In tetraploid Ae. ventricosa, both 4D and 4M could be painted; however, the signal intensity of 4M was less compared with 4D. No painted chromosome was observed for the other alien species. This indicated that the relationship among D/M/U was closer than that among D/A/B as well as D with genomes H/R/S
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