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ArticleScientific reports2020

Genome-wide SNP identification in Prunus rootstocks germplasm collections using Genotyping-by-Sequencing: phylogenetic analysis, distribution of SNPs and prediction of their effect on gene function.

Verónica Guajardo, Simón Solís, Rubén Almada, Christopher Saski, Ksenija Gasic, María Ángeles Moreno

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In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Genomic Variability Survey inInternational journal of molecular sciences · 2024
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  8. The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Verónica GuajardoCentro de Estudios Avanzados en Fruticultura (CEAF), Rengo, Chile.
Simón SolísCentro de Estudios Avanzados en Fruticultura (CEAF), Rengo, Chile.
Rubén AlmadaCentro de Estudios Avanzados en Fruticultura (CEAF), Rengo, Chile.
Christopher SaskiDepartment of Plant and Environmental Sciences, Clemson University, Clemson, SC, 29634, USA.
Ksenija GasicDepartment of Plant and Environmental Sciences, Clemson University, Clemson, SC, 29634, USA.
María Ángeles MorenoDepartment of Pomology, Estación Experimental de Aula Dei-CSIC, 50059, Zaragoza, Spain. mmoreno@eead.csic.es.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genotyping-by-Sequencing (GBS) was applied in a set of 53 diploid Prunus rootstocks and five scion cultivars from three subgenera (Amygdalus, Prunus and Cerasus) for genome-wide SNP identification and to assess genetic diversity of both Chilean and Spanish germplasm collections. A group of 45,382 high quality SNPs (MAF >0.05; missing data <5%) were selected for analysis of this group of 58 accessions. These SNPs were distributed in genic and intergenic regions in the eight pseudomolecules of the peach genome (Peach v2.0), with an average of 53% located in exonic regions. The genetic diversity detected among the studied accessions divided them in three groups, which are in agreement with their current taxonomic classification. SNPs were classified based on their putative effect on annotated genes and KOG analysis was carried out to provide a deeper understanding of the function of 119 genes affected by high-impact SNPs. Results demonstrate the high utility for Prunus rootstocks identification and studies of diversity in Prunus species. Also, given the high number of SNPs identified in exonic regions, this strategy represents an important tool for finding candidate genes underlying traits of interest and potential functional markers for use in marker-assisted selection.

Indexed as

Genome-Wide Association StudyGene Expression Regulation, PlantGenome, PlantGenotyping TechniquesHigh-Throughput Screening AssaysPhylogenyPlant RootsPolymorphism, Single NucleotidePrunusPrunus persicaSeed BankSequence Analysis, DNA

Identifiers

PMID32001784
PMCPMC6992769

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.