ArticleVirology journal2020
Analysis of viral diversity in stool samples from infants and children with acute gastroenteritis in Kuwait using Metagenomics approach.
Article in Virology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 39 citations in OpenAlex.
- Epidemiology and genetic variation of acute viral gastroenteritis in children under five years in the Middle East (2020-2025): a systematic review and meta-analysis.BMC infectious diseases · 2026Pooled it
- Global Status of Bufavirus, Cosavirus, and Saffold Virus in Gastroenteritis: A Systematic Review and Meta-Analysis.Frontiers in medicine · 2021Pooled it
- Understanding the bacteriome, phageome and phage-associated bacteriome in healthy Vietnamese children under two years of age.Archives of microbiology · 2026Article
- Prevalence of pathogens associated with neonatal gastrointestinal infections: a systematic review and meta-analysis.Gut pathogens · 2025Review
- Detection and complete genome sequence analysis of human adenovirus in children with acute diarrhea in Yunnan, China, 2015-2021.Archives of virology · 2024Article
- Screening for viral pathogens in the gastrointestinal tract from cases of sudden unexpected death in infancy at the Tygerberg Medico-legal Mortuary.Virology journal · 2023Article
- Gastrointestinal Infection in South African Children under the Age of 5 years: A Mini Review.Gastroenterology research and practice · 2023Review
- Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome.Microorganisms · 2022Review
- Human Mastadenovirus Infections in Children: A Review of the Current Status in the Arab World in the Middle East and North Africa.Children (Basel, Switzerland) · 2022Review
- Gut virome in early life: origins and implications.Current opinion in virology · 2022Review
- Therapeutic effectiveness of green tea leaf extract on clinical symptoms in children suffering viral gastroenteritis: A randomized clinical trial.European journal of translational myology · 2022Article
- Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea.Virologica Sinica · 2022Article
- Viruses associated with ill health in wild chimpanzees.American journal of primatology · 2022Article
- Acute Infectious Gastroenteritis: The Causative Agents, Omics-Based Detection of Antigens and Novel Biomarkers.Children (Basel, Switzerland) · 2021Review
- Rotavirus and illness severity in children presenting with acute gastroenteritis at the primary care out-of-hours service.The European journal of general practice · 2021Article
- Composition of Eukaryotic Viruses and Bacteriophages in Individuals with Acute Gastroenteritis.Viruses · 2021Article
- Epidemiology of Human Bocavirus in the Middle East and North Africa: Systematic Review.Pathogens (Basel, Switzerland) · 2021Review
- Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016.Archives of virology · 2021Article
- Human Protoparvovirus DNA and IgG in Children and Adults with and without Respiratory or Gastrointestinal Infections.Viruses · 2021Article
- Demography, life-history trade-offs, and the gastrointestinal virome of wild chimpanzees.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrent molecular target-dependent methods are used to detect only known viruses. However, metagenomics based on next-generation sequencing (NGS) technique is a target-independent assay that enables simultaneous detection and genomic characterisation of all microorganisms present in a sample. In this study, we aimed to develop a metagenomics approach using NGS to identify and characterise viruses in stool samples from infants and children with Acute Gastroenteritis (AGE) in Kuwait.
methodsWe have investigated 84 stool samples from infants and children aged one month to ten years old with signs and symptoms of gastroenteritis who attended Mubarak Al-Kabeer and Al-Amiri hospitals in Kuwait from January to December 2017. A metagenomics approach using NGS to characterise viruses in clinical samples was used. Also, the commercial Real-Time PCR assay was used to detect viruses causing gastroenteritis.
resultsMetagenomics analysis revealed an average of 280,768 reads in which 5% of the reads were derived from viruses. The analysis of viral sequences verified that single infection of human adenovirus was the leading cause of gastroenteritis among infants and children, which was detected in 23.2% of the patients, followed by a mixed infection of human adenovirus and other viruses, which was detected in 20.9% of patients. Also, the newly discovered viruses known to cause gastroenteritis were detected, such as astrovirus MLB2, primate bocaparvovirus-1, Aichivirus A, cardiovirus, parechovirus A, astrovirus VA4, cosavirus-F, and bufavirus-3. Our results showed 71% agreement (k = 0.445, P = 0.000) between multiplex Real-Time PCR, which is used as a routine diagnostic test and metagenomics approach in the detection of viruses causing gastroenteritis in clinical samples.
conclusionDespite the difficulties in sample preparation and analysis process, we showed that metagenomics approach is a powerful and promising tool for the detection and characterisation of different viruses in clinical samples.
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