ArticleJournal of dental research2020
Modeling Normal and Dysbiotic Subgingival Microbiomes: Effect of Nutrients.
Article in Journal of dental research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Nutritional pressure from serum amplifies dysbiotic features in an oral microbiome synthetic community.The ISME journal · 2026Article
- Examining Oral Microbial Communities at the Gingival Interface During Health and Inflammatory Diseases.Advances in experimental medicine and biology · 2026Review
- The Impact of Serum on a Complex Synthetic Community Model of the Subgingival Microbiome.bioRxiv : the preprint server for biology · 2025Article
- Ex vivo oral biofilm model for rapid screening of antimicrobial agents including natural cranberry polyphenols.Scientific reports · 2025Article
- The effect of dental material type and masticatory forces on periodontitis-derived subgingival microbiomes.Biofilm · 2024Article
- Investigation of a novel biofilm model close to the original oral microbiome.Applied microbiology and biotechnology · 2024Article
- Construction of a versatile in vitro cultivation screening platform using human oral microbiota.Environmental microbiology reports · 2024Article
- Multi-Omics Data Integration Reveals Key Variables Contributing to Subgingival Microbiome Dysbiosis-Induced Inflammatory Response in a Hyperglycemic Microenvironment.International journal of molecular sciences · 2023Article
- Food, gut barrier dysfunction, and related diseases: A new target for future individualized disease prevention and management.Food science & nutrition · 2023Review
- The Role and Involvement of Stem Cells in Periodontology.Biomedicines · 2023Review
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular ecological studies.FEMS microbiology reviews · 2023Review
- Optimization of conditions forFrontiers in microbiology · 2022Article
- Saliva-derived microcosm biofilms grown on different oral surfaces in vitro.NPJ biofilms and microbiomes · 2021Article
- Transcriptomic phases of periodontitis lesions using the nonhuman primate model.Scientific reports · 2021Article
- Modified SHI medium supports growth of a disease-state subgingival polymicrobial community in vitro.Molecular oral microbiology · 2021Article
- Manipulation of Saliva-Derived Microcosm Biofilms To Resemble Dysbiotic Subgingival Microbiota.Applied and environmental microbiology · 2021Article
- Oral Microbiome and Gingival Gene Expression of Inflammatory Biomolecules With Aging and Periodontitis.Frontiers in oral health · 2021Article
- Periodontal dysbiosis associates with reduced CSF Aβ42 in cognitively normal elderly.Alzheimer's & dementia (Amsterdam, Netherlands) · 2021Article
- Future directions for studying resilience of the oral ecosystem.British dental journal · 2020Article
- Probiotics alter biofilm formation and the transcription ofJournal of oral microbiology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Screening for microbiome modulators requires availability of a high-throughput in vitro model that replicates subgingival dysbiosis and normobiosis, with a tool to measure microbial dysbiosis. Here, we tested various formulations to grow health- and periodontitis-associated subgingival microbiomes in parallel, and we describe a new subgingival dysbiosis index. Subgingival plaque samples pooled from 5 healthy subjects and, separately, 5 subjects with periodontitis were used to inoculate a Calgary Biofilm Device containing saliva-conditioned, hydroxyapatite-coated pegs. Microbiomes were grown for 7 d on either nutrient-rich media-including a modification of SHI medium, brain-heart infusion (BHI) supplemented with hemin and vitamin K, and a blend of SHI and BHI, each at 3 sucrose concentrations (0%, 0.05% and 0.1%)-or nutrient-limited media (saliva with 5%, 10%, or 20% inactivated human serum). The microbiomes were assessed for biomass, viability, and 16S rRNA profiles. In addition to richness and diversity, a dysbiosis index was calculated as the ratio of the sum of relative abundances of disease-associated species to that of health-associated species. The supplemented BHI and blend of SHI and BHI resulted in the highest biomass, whereas saliva-serum maximized viability. Distinct groups of bacteria were enriched in the different media. Regardless of medium type, the periodontitis-derived microbiomes showed higher species richness and alpha diversity and clustered with their inoculum separate from the health-derived microbiomes. Microbiomes grown in saliva-serum showed the highest species richness and the highest similarity to the clinical inocula in both health and disease. However, inclusion of serum reduced alpha diversity and increased dysbiosis in healthy microbiomes in a dose-dependent manner, mainly due to overenrichment of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.