ArticlePloS one2020
Construction of a RFP-lacZα bicistronic reporter system and its application in lead biosensing.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
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- PlomBOX: a low cost bioassay for the sensitive detection of lead in drinking water.Communications engineering · 2025Article
- Systematic evaluation of pigment-based whole-cell lead biosensors: challenges in genetic circuit engineering and critical considerations for background noise control.Frontiers in bioengineering and biotechnology · 2025Article
- A Chimeric ORF Fusion Phenotypic Reporter forJournal of fungi (Basel, Switzerland) · 2024Article
- Biosynthesis of Indigo Dyes and Their Application in Green Chemical and Visual Biosensing for Heavy Metals.ACS omega · 2024Article
- Synthetic bacteria designed using ars operons: a promising solution for arsenic biosensing and bioremediation.World journal of microbiology & biotechnology · 2024Review
- Anthocyanin biosynthetic pathway switched by metalloregulator PbrR to enable a biosensor for the detection of lead toxicity.Frontiers in microbiology · 2022Article
- Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review.World journal of microbiology & biotechnology · 2021Review
- Indigoidine biosynthesis triggered by the heavy metal-responsive transcription regulator: a visual whole-cell biosensor.Applied microbiology and biotechnology · 2021Article
- Development of a bioavailable Hg(II) sensing system based on MerR-regulated visual pigment biosynthesis.Scientific reports · 2021Article
- In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook.World journal of microbiology & biotechnology · 2021Review
- Development of Cadmium Multiple-Signal Biosensing and Bioadsorption Systems Based on ArtificialFrontiers in bioengineering and biotechnology · 2021Article
- Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System.Frontiers in microbiology · 2021Article
- Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.PloS one · 2021Article
- Genetic control of violacein biosynthesis to enable a pigment-based whole-cell lead biosensor.RSC advances · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The combination of a fluorescent reporter and enzymatic reporter provides a flexible and versatile way for the study of diverse biological processes, such as the detection of transcription and translation. Thus, there is an urgent need to develop this novel bifunctional reporter system. This study reports the design, construction, and validation of a new dicistronic mCherry-lacZα reporter system by artificial lac operon and pbr operon models in lacZM15-producing E. coli. It allows two reporter genes to be co-transcribed into a dicistronic mRNA strand, followed by coupled expression of mCherry and lacZα. In artificial lac operons, expression of the downstream lacZα was demonstrated to be positively related to expression of the upstream ORF. In artificial pbr operons, compared with the insertion of downstream full-length lacZ, the insertion of downstream lacZα exerted a slight effect on the response from the upstream mCherry. Furthermore, the downstream lacZα reporter showed stronger response to Pb(II) than the downstream full-length lacZ. Importantly, the response sensitivity of downstream lacZα was still higher than that of upstream mCherry in a dual RFP-lacZα reporter construct. The highly efficient expression profile of the reporter lacZα peptide makes it a preferred downstream reporter in polycistronic constructs. This novel bifunctional reporter system offers a robust tool for biological studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.