ArticlePloS one2020
Leptospiral shedding and seropositivity in shelter dogs in the Cumberland Gap Region of Southeastern Appalachia.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Pathogen Detection and Resistome Analysis in Healthy Shelter Dogs Using Whole Metagenome Sequencing.Pathogens (Basel, Switzerland) · 2025Article
- Detection of leptospiral antibodies and DNA in freshwater fish.Frontiers in veterinary science · 2025Article
- Spatiotemporal analysis and clinico-epidemiological study for seroprevalence of canine leptospirosis.Veterinary research communications · 2024Article
- Diagnostic Pathways ofPathogens (Basel, Switzerland) · 2024Article
- Feline leptospirosis prevalence worldwide: A systematic review and meta-analysis of diagnostic approaches.Veterinary world · 2024Article
- Seroprevalence of pathogenicFrontiers in veterinary science · 2024Review
- A Canine Leptospirosis Clinical Case Due toVeterinary sciences · 2023Article
- Effect of Vaccination againstAnimals : an open access journal from MDPI · 2022Article
- Use of machine-learning algorithms to aid in the early detection of leptospirosis in dogs.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- A case-control study of agricultural and behavioral factors associated with leptospirosis in Vietnam.BMC infectious diseases · 2022Article
- Role of Diagnostics in Epidemiology, Management, Surveillance, and Control of Leptospirosis.Pathogens (Basel, Switzerland) · 2022Review
- Persistent High Leptospiral Shedding by Asymptomatic Dogs in Endemic Areas Triggers a Serious Public Health Concern.Animals : an open access journal from MDPI · 2021Article
- Seroprevalence and Associated Risk Factors of CanineVeterinary medicine (Auckland, N.Z.) · 2021Article
- Detection ofVeterinary world · 2020Article
- Article
- Updated ACVIM consensus statement on leptospirosis in dogs.Journal of veterinary internal medicineArticle
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeptospirosis, caused by pathogenic Leptospira spp., is a zoonotic infection that affects humans, dogs and many other mammalian species. Virtually any mammalian species can act as asymptomatic reservoir, characterized by chronic renal carriage and shedding of a host-adapted leptospiral serovar. Environmental contamination by chronic shedders results in acquisition of infection by humans and susceptible animals.
methodsIn this study, we investigated if clinically normal shelter dogs and cats harbor leptospires in their kidneys by screening urine samples for the presence of leptospiral DNA by a TaqMan based-quantitative PCR (qPCR) that targets pathogen-associated lipl32 gene. To identify the infecting leptospiral species, a fragment of leptospiral rpoB gene was PCR amplified and sequenced. Additionally, we measured Leptospira-specific serum antibodies using the microscopic agglutination test (MAT), a gold standard in leptospiral serology.
resultsA total of 269 shelter animals (219 dogs and 50 cats) from seven shelters located in the tri-state area of western Virginia, eastern Tennessee, and southeastern Kentucky were included in this study. All cats tested negative by both qPCR and MAT. Of the 219 dogs tested in the study, 26/198 (13.1%, 95% CI: 8.4-17.8%) were positive for leptospiral DNA in urine by qPCR and 38/211 (18.0%, 95% CI: 12.8-23.2%) were seropositive by MAT. Twelve dogs were positive for both qPCR and MAT. Fourteen dogs were positive by qPCR but not by MAT. Additionally, leptospiral rpoB gene sequencing from a sub-set of qPCR-positive urine samples (n = 21) revealed L. interrogans to be the leptospiral species shed by dogs.
conclusionsThese findings have significant implications regarding animal and public health in the Cumberland Gap Region and possibly outside where these animals may be adopted.
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