ArticleOdontology2020
Acute cytotoxic effects of silica microparticles used for coating of plastic blood-collection tubes on human periosteal cells.
Article in Odontology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Use of platelet-rich fibrin for the treatment of gingival recessions: a systematic review and meta-analysis.Clinical oral investigations · 2020Pooled it
- Effectiveness of using platelet-rich fibrin to increase keratinized tissue around the tooth in the modified apical reposition flap method: A split-mouth, randomized controlled trial.Clinical and experimental dental research · 2023Trial
- Use of Platelet-Rich Fibrin and Platelet-Rich Plasma as Delivery Systems for Natural Compounds: A Systematic Review.Materials (Basel, Switzerland) · 2026Review
- Effects of Nano-Hydroxyapatite-Coated PRF on Gingiva-Derived Mesenchymal Stem Cells: In Vitro Study.International journal of molecular sciences · 2026Article
- In vitro investigation of the effect of laser on platelet-rich fibrins.BMC oral health · 2026Article
- Safety and Feasibility of Extended Platelet-Rich Fibrin as a Solo Barrier Membrane for Ridge Preservation: A Case Series.Clinical and experimental dental research · 2026Article
- Platelet-Rich Fibrin and Its Derivatives as Adjuncts to Dental Implant Osseointegration: A Scoping Review.International journal of dentistry · 2026Review
- The Impact of Centrifugation Devices and Collection Tubes on Fibrin Characteristics and Growth Factor Release Under High- and Low-Speed Protocols.Dentistry journal · 2025Article
- Comparative Analysis of Local Application of Titanium-Platelet Rich Fibrin (T-PRF) and Leukocyte-Platelet Rich Fibrin (L-PRF) in Bone Defect Healing: A Micro-CT and Histopathological Study in Rabbit Models.Microscopy research and technique · 2025Article
- Mitochondrial Transfer from Human Platelets to Rat Dental Pulp-Derived Fibroblasts in the 2D In Vitro System: Additional Implication in PRP Therapy.International journal of molecular sciences · 2025Article
- Differences between first- and second-generation autologous platelet concentrates.Periodontology 2000 · 2025Review
- Lyophilized horizontal platelet rich fibrin promotes the healing of infected burns/wounds by modulating macrophage polarization and fibroblast migration.Frontiers in bioengineering and biotechnology · 2025Article
- Effect of silica-sprayed collection tubes on synovial fluid bacterial culture.Veterinary medicine and science · 2024Article
- RE: Fibrin Network and Platelets Densities in Platelet‑Rich Fibrin (PRF) Membranes Produced from Plastic Tubes Without Additives-A New Approach to PRF Clinical Use.Journal of maxillofacial and oral surgery · 2024Article
- Fibrin Network and Platelets Densities in Platelet-Rich Fibrin (PRF) Membranes Produced from Plastic Tubes Without Additives: A New Approach to PRF Clinical Use.Journal of maxillofacial and oral surgery · 2024Article
- Understanding Solid-Based Platelet-Rich Fibrin Matrices in Oral and Maxillofacial Surgery: An Integrative Review of the Critical Protocol Factors and Their Influence on the Final Product.Medicina (Kaunas, Lithuania) · 2023Review
- Heterochronic Plasma Transfer: Experimental Design, Considerations, and Technical Challenges.Rejuvenation research · 2023Review
- Platelet-rich fibrin improves the osteoneogenesis in non-critical defects in calvaria: a histological and histometric study.Acta cirurgica brasileira · 2023Article
- Responses of promyelocytic leukemia HL60 cells as an inflammatory cell lineage model to silica microparticles used to coat blood collection tubes.International journal of implant dentistry · 2022Article
- Environmental risks of polymer materials from disposable face masks linked to the COVID-19 pandemic.The Science of the total environment · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Because of its simple operation, platelet-rich fibrin (PRF) is becoming more popular than the original form, platelet-rich plasma (PRP), in regenerative dentistry. PRF preparation requires plain glass blood-collection tubes, but not either anticoagulants or coagulation factors. However, such glass tubes designed for laboratory testing are no longer commercially available. Although several glass tubes specifically designed for PRF preparation are available, many clinicians prefer to obtain stably supplied substitutes, such as silica-coated plastic tubes produced by major medical device companies. The quality of PRF prepared by silica-coated tubes has not been assessed and we previously reported significant contamination of silica microparticles in the resulting PRF matrix and alerted clinicians against the use for PRF preparation. To further assess the biosafety of the silica microparticles, we presently examined their effects on human normal periosteal cells derived from alveolar bone. The periosteal cells were obtained from explant cultures of small periosteal tissues obtained from healthy donors. Silica microparticles were obtained from silica-coated tubes and added to cell cultures. Cellular responses were monitored using a tetrazolium assay, phase-contract inverted microscopy, an immunofluorescence method, and scanning electron microscopy. Silica microparticles adsorbed onto the cell surface with seemingly high affinity and induced apoptosis, resulting in significant reduction of cell proliferation and viability. These findings suggest that silica microparticles contained in plastic tubes for the purpose of blood coagulation are hazardous for various cell types around sites where silica-contaminated PRF matrices are implanted.
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Registered trials
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