ArticleNature protocols2020
Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.
Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Real-time single-particle imaging of functional lungs reveals mosaic-like patterns of aerosol deposition in alveoli.Nature biomedical engineering · 2026Article
- Finding and filling the knowledge gaps in mechanisms of T cell-mediated TB immunity to inform vaccine design.Nature reviews. Immunology · 2025Review
- Molecular probes for in vivo optical imaging of immune cells.Nature biomedical engineering · 2025Review
- Neutrophil adhesion to vessel walls impairs pulmonary circulation in COVID-19 pathology.Nature communications · 2025Article
- TWINKLE: An open-source two-photon microscope for teaching and research.bioRxiv : the preprint server for biology · 2025Article
- Article
- LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages.Nature communications · 2024Article
- Review
- Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8Immunity · 2024Article
- Development of a Mouse-Adapted Reporter SARS-CoV-2 as a Tool for Two-Photon In Vivo Imaging.Viruses · 2024Article
- Techniques for Measurement of Serotonin: Implications in Neuropsychiatric Disorders and Advances in Absolute Value Recording Methods.ACS chemical neuroscience · 2023Review
- Real-time imaging of dynamic tissues.Nature methods · 2023Article
- Crystal ribcage: a platform for probing real-time lung function at cellular resolution.Nature methods · 2023Article
- Stabilized Window for Intravital Imaging of the Murine Pancreas.Journal of visualized experiments : JoVE · 2023Article
- From static to dynamic: live observation of the support system after ischemic stroke by two photon-excited fluorescence laser-scanning microscopy.Neural regeneration research · 2023Review
- Infection with Influenzavirus A in a murine model induces epithelial bronchial lesions and distinct waves of innate immune-cell recruitment.Frontiers in immunology · 2023Article
- NLRP3 inflammasome activation in sensory neurons promotes chronic inflammatory and osteoarthritis pain.Immunotherapy advances · 2023Article
- Multi-Color Two-Photon Microscopic Imaging Based on a Single-Wavelength Excitation.Biosensors · 2022Article
- Imaging viral infection in vivo to gain unique perspectives on cellular antiviral immunity.Immunological reviews · 2022Review
- Review on Optical Imaging Techniques for Multispectral Analysis of Nanomaterials.Nanotheranostics · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
Abstract
In vivo two-photon imaging is a valuable technique for studies of viral pathogenesis and host responses to infection in vivo. In this protocol, we describe a methodology for analyzing influenza virus-infected lung in vivo by two-photon imaging microscopy. We describe the surgical procedure, how to stabilize the lung, and an approach to analyzing the data. Further, we provide a database of fluorescent dyes, antibodies, and reporter mouse lines that can be used in combination with a reporter influenza virus (Color-flu) for multicolor analysis. Setup of this model typically takes ~30 min and enables the observation of influenza virus-infected lungs for >4 h during the acute phase of the inflammation and at least 1 h in the lethal phase. This imaging system, which we termed two-photon IMPRESS (imaging pathophysiology research system), is broadly applicable to analyses of other respiratory pathogens and reveals disease progression at the cellular level in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.