ArticleNature communications2020
Single-cell transcriptomics identifies CD44 as a marker and regulator of endothelial to haematopoietic transition.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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Who cites it
58 citing papers in PubMed, 102 citations in OpenAlex.
- Article
- From matrix to nucleus: CD44 as a central regulator of inflammaging, autophagy, and tissue homeostasis.Biogerontology · 2026Review
- Mechanometabolism instructs hematopoietic stem cell specification.The Journal of experimental medicine · 2026Article
- Apelin signaling acts as a molecular switch between endothelial and hematopoietic stem cell fates.EMBO reports · 2026Article
- MEN1 Promotes Ferroptosis by Disrupting CD44 Alternative Splicing to Suppress Lung Cancer.Cancer research · 2026Article
- Bloodhounds chasing the origin of blood cells.Trends in cell biology · 2025Review
- CBKMR: A Copula-based Bayesian Kernel Machine Regression Framework for Optimal Marker Detection in Omics Data.bioRxiv : the preprint server for biology · 2025Article
- Developmental regulation of endothelial-to-hematopoietic transition from induced pluripotent stem cells.Stem cell reports · 2025Article
- Article
- Conversion of placental hemogenic endothelial cells to hematopoietic stem and progenitor cells.Cell discovery · 2025Article
- Unraveling hematopoietic stem cell ontogeny through single-cell multi-omics approaches.Frontiers in cell and developmental biology · 2025Review
- Article
- Therapeutic Effects of Coenzyme Q10 in the Treatment of Ischemic Stroke.Current nutrition reports · 2024Review
- NR4A1 and NR4A2 orphan nuclear receptors regulate endothelial-to-hematopoietic transition in mouse hematopoietic stem cell specification.Development (Cambridge, England) · 2024Article
- Prolactin Drives Iron Release from Macrophages and Uptake in Mammary Cancer Cells through CD44.International journal of molecular sciences · 2024Article
- Mitochondrial permeability transition dictates mitochondrial maturation upon switch in cellular identity of hematopoietic precursors.Communications biology · 2024Article
- Systematic single-cell analysis reveals dynamic control of transposable element activity orchestrating the endothelial-to-hematopoietic transition.BMC biology · 2024Article
- FLI1 is associated with regulation of DNA methylation and megakaryocytic differentiation in FPDMM caused by a RUNX1 transactivation domain mutation.Scientific reports · 2024Article
- CD32 captures committed haemogenic endothelial cells during human embryonic development.Nature cell biology · 2024Article
- Article
Corrections and comments
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Authors and funding
15 authors at 8 institutions in 6 countries.
Funding
Abstract
The endothelial to haematopoietic transition (EHT) is the process whereby haemogenic endothelium differentiates into haematopoietic stem and progenitor cells (HSPCs). The intermediary steps of this process are unclear, in particular the identity of endothelial cells that give rise to HSPCs is unknown. Using single-cell transcriptome analysis and antibody screening, we identify CD44 as a marker of EHT enabling us to isolate robustly the different stages of EHT in the aorta-gonad-mesonephros (AGM) region. This allows us to provide a detailed phenotypical and transcriptional profile of CD44-positive arterial endothelial cells from which HSPCs emerge. They are characterized with high expression of genes related to Notch signalling, TGFbeta/BMP antagonists, a downregulation of genes related to glycolysis and the TCA cycle, and a lower rate of cell cycle. Moreover, we demonstrate that by inhibiting the interaction between CD44 and its ligand hyaluronan, we can block EHT, identifying an additional regulator of HSPC development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.