ArticlemSphere2020
Influence of Substrate Concentration on the Culturability of Heterotrophic Soil Microbes Isolated by High-Throughput Dilution-to-Extinction Cultivation.
Article in mSphere, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 56 citations in OpenAlex.
- The gut-tumor connection: the role of microbiota in cancer progression and treatment strategies.Journal of advanced research · 2026Review
- Transcriptional and metabolic stasis define desiccation-induced dormancy in the soil bacteriummSystems · 2026Article
- Microecological insight into the microbial structure, key cellulolytic community, and microbial interaction during cellulose degradation in high-diversity and low-diversity communities.Applied and environmental microbiology · 2026Article
- Variations in deep-sea microbial composition and assembly mechanisms under different culture strategies.Frontiers in microbiology · 2026Article
- Effect of baby food supplement on the growth and metabolite of lactic acid bacteria.BMC microbiology · 2025Article
- Bringing the uncultivated microbial majority of freshwater ecosystems into culture.Nature communications · 2025Article
- A diffusion-based integrative approach for culturing previously uncultured bacteria from marine sediments.Marine life science & technology · 2025Article
- Investigation of co-existing bacteria in platelets by employing long-term culturomics and metagenomics.Frontiers in cellular and infection microbiology · 2025Article
- High-quality PacBio draft genome sequences of 17 free-living Bradyrhizobium and four related Nitrobacteraceae strains isolated from arid soils in the Santa Catalina Mountains of Southern Arizona.Access microbiology · 2025Article
- Draft genome sequences ofMicrobiology resource announcements · 2024Article
- Soil microbiome engineering for sustainability in a changing environment.Nature biotechnology · 2023Review
- Expanding success in the isolation of abundant marine bacteria after reduction in grazing and viral pressure and increase in nutrient availability.Microbiology spectrum · 2023Article
- Experimentally determined traits shape bacterial community composition one and five years following wildfire.Nature ecology & evolution · 2023Article
- Hyperarid soil microbial community response to simulated rainfall.Frontiers in microbiology · 2023Article
- Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons.Frontiers in microbiology · 2023Review
- Nontuberculous Mycobacteria as Sapronoses: A Review.Microorganisms · 2022Review
- Oligotrophy vs. copiotrophy in an alkaline and saline habitat of Lonar Lake.Frontiers in microbiology · 2022Article
- A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria.Frontiers in microbiology · 2022Article
- Efficient dilution-to-extinction isolation of novel virus-host model systems for fastidious heterotrophic bacteria.The ISME journal · 2021Article
- Innovations to culturing the uncultured microbial majority.Nature reviews. Microbiology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The vast majority of microbes inhabiting oligotrophic shallow subsurface soil environments have not been isolated or studied under controlled laboratory conditions. In part, the challenges associated with isolating shallow subsurface microbes may persist because microbes in deeper soils are adapted to low nutrient availability or quality. Here, we use high-throughput dilution-to-extinction culturing to isolate shallow subsurface microbes from a conifer forest in Arizona, USA. We hypothesized that the concentration of heterotrophic substrates in microbiological growth medium would affect which microbial taxa were culturable from these soils. To test this, we diluted cells extracted from soil into one of two custom-designed defined growth media that differed by 100-fold in the concentration of amino acids and organic carbon. Across the two media, we isolated a total of 133 pure cultures, all of which were classified as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.