ArticleLangmuir : the ACS journal of surfaces and colloids2020
Nonspherical Coacervate Shapes in an Enzyme-Driven Active System.
Article in Langmuir : the ACS journal of surfaces and colloids, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Article
- Enzyme-Induced Transition of the Morphology of Polyelectrolyte Complexes.Biomacromolecules · 2026Article
- Defining the role of RNase E in the mycobacterial degradosome-like network.bioRxiv : the preprint server for biology · 2025Article
- Converging frontiers in biomolecular condensate and synthetic cell research.npj biomedical innovations · 2025Review
- Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction.Nature communications · 2025Article
- Pyrite as a catalyst for the emergence of multiphase primitive cells.Frontiers in microbiology · 2025Article
- Enzymatic Reaction Network-Driven Polymerization-Induced Transient Coacervation.Angewandte Chemie (International ed. in English) · 2024Article
- Shape transformations in peptide-DNA coacervates driven by enzyme-catalyzed deacetylation.Soft matter · 2024Article
- Harnessing Competitive Interactions to Regulate Supramolecular "Micelle-Droplet-Fiber" Transition and Reversibility in Water.Journal of the American Chemical Society · 2024Article
- Temporally controlled multistep division of DNA droplets for dynamic artificial cells.Nature communications · 2024Article
- Did the exposure of coacervate droplets to rain make them the first stable protocells?Science advances · 2024Article
- Article
- Designing negative feedback loops in enzymatic coacervate droplets.Chemical science · 2023Article
- ATP:MgBiophysical journal · 2022Article
- Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases.Diagnostics (Basel, Switzerland) · 2022Review
- Growth, replication and division enable evolution of coacervate protocells.Chemical communications (Cambridge, England) · 2022Review
- Article
- Fuel-driven macromolecular coacervation in complex coacervate core micelles.Chemical science · 2022Article
- CRISPR-based DNA and RNA detection with liquid-liquid phase separation.Biophysical journal · 2021Article
- Enzymatic degradation of liquid droplets of DNA is modulated near the phase boundary.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coacervates are polymer-rich droplets that form through liquid-liquid phase separation in polymer solutions. Liquid-liquid phase separation and coacervation have recently been shown to play an important role in the organization of biological systems. Such systems are highly dynamic and under continuous influence of enzymatic and chemical processes. However, it is still unclear how enzymatic and chemical reactions affect the coacervation process. Here, we present and characterize a system of enzymatically active coacervates containing spermine, RNA, free nucleotides, and the template independent RNA (de)polymerase PNPase. We find that these RNA coacervates display transient nonspherical shapes, and we systematically study how PNPase concentration, UDP concentration, and temperature affect coacervate morphology. Furthermore, we show that PNPase localizes predominantly into the coacervate phase and that its depolymerization activity in high-phosphate buffer causes coacervate degradation. Our observations of nonspherical coacervate shapes may have broader implications for the relationship between (bio)chemical activity and coacervate biology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.