ArticleAnalytical and bioanalytical chemistry2020
Development and bioanalytical method validation of an LC-MS/MS assay for simultaneous quantitation of 2-alkyl-4(1H)-quinolones for application in bacterial cell culture and lung tissue.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Calprotectin protectsmSystems · 2025Article
- LC-MS/MS Determination of Quorum Sensing Molecules in Plasma from Burn Patients with Septic Shock Sustained by Acinetobacter Baumannii.Antibiotics (Basel, Switzerland) · 2025Article
- Effect of Micro-Patterned Mucin on Quinolone and Rhamnolipid Profiles of MucoidACS infectious diseases · 2023Article
- The OmpA of commensal Escherichia coli of CRC patients affects apoptosis of the HCT116 colon cancer cell line.BMC microbiology · 2022Article
- Alkyl-Quinolones derivatives as potential biomarkers for Pseudomonas aeruginosa infection chronicity in Cystic Fibrosis.Scientific reports · 2021Article
- Biological and clinical significance of quorum sensing alkylquinolones: current analytical and bioanalytical methods for their quantification.Analytical and bioanalytical chemistry · 2021Review
- Targeted and untargeted quantification of quorum sensing signalling molecules in bacterial cultures and biological samples via HPLC-TQ MS techniques.Analytical and bioanalytical chemistry · 2021Article
- Bacterial Alkyl-4-quinolones: Discovery, Structural Diversity and Biological Properties.Molecules (Basel, Switzerland) · 2020Review
- Static Growth Promotes PrrF and 2-Alkyl-4(1Journal of bacteriology · 2020Article
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Authors and funding
6 authors.
Funding
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that produces numerous exoproducts during infection that help it evade the host immune system and procure nutrients from the host environment. Among these products are a family of secreted 2-alkyl-4(1H)-quinolone metabolites (AQs), which exhibit a range of biological activities. Here, we describe the validation of a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for quantifying multiple AQ congeners in complex biological matrices. The assay was validated for selectivity, sensitivity, linearity, accuracy, precision, carryover, dilution integrity, recovery, matrix effects, and various aspects of stability (freeze-thaw, bench-top, long-term storage, and autosampler/post-preparative). Using authentic standards for 6 distinct AQ congeners, we report accurate quantitation within a linear range between 25 and 1000 nmol/L for all of the validated AQ standards. This method was successfully applied to quantify AQ concentrations in P. aeruginosa cell culture and in the lungs of mice infected with P. aeruginosa. Further, we confirmed the presence of unsaturated forms of several AQ congeners in cell culture. Graphical abstract.
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Registered trials
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