ArticleNucleic acids research2020
Generation of pre-tRNAs from polycistronic operons is the essential function of RNase P in Escherichia coli.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- pCODE tRNA Expression Plasmids Compensate for Rare Codons in Recombinant Protein Production.ACS synthetic biology · 2026Article
- tRNA-modifying enzymes in bacterial stress adaptation.Open biology · 2025Review
- Bacterial/archaeal protein-only RNase P: complementation inRNA (New York, N.Y.) · 2025Article
- The DnaJK chaperone ofmBio · 2025Article
- Suppression of theRNA (New York, N.Y.) · 2024Article
- A minimal RNA substrate with dual fluorescent probes enables rapid kinetics and provides insight into bacterial RNase P active site interactions.RSC chemical biology · 2024Article
- Interactors and effects of overexpressing YlxR/RnpM, a conserved RNA binding protein in cyanobacteria.RNA biology · 2024Article
- Highly efficient multiplex base editing: One-shot deactivation of eight genes inSynthetic and systems biotechnology · 2023Article
- Tyrosine phosphorylation controlled poly(A) polymerase I activity regulates general stress response in bacteria.Life science alliance · 2023Article
- A tRNA-derived fragment present in E. coli OMVs regulates host cell gene expression and proliferation.PLoS pathogens · 2022Article
- Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P.Nature communications · 2022Article
- Type III-A CRISPR systems as a versatile gene knockdown technology.RNA (New York, N.Y.) · 2022Article
- Synthetic riboswitches for the analysis of tRNA processing by eukaryotic RNase P enzymes.RNA (New York, N.Y.) · 2022Article
- The C nucleotide at the mature 5' end of the Escherichia coli proline tRNAs is required for the RNase E cleavage specificity at the 3' terminus as well as functionality.Nucleic acids research · 2022Article
- Regulation of mRNA decay inCritical reviews in biochemistry and molecular biology · 2022Article
- Inactivation of RNase P in Escherichia coli significantly changes post-transcriptional RNA metabolism.Molecular microbiology · 2022Article
- Transgenesis of mammalian PABP reveals mRNA polyadenylation as a general stress response mechanism in bacteria.iScience · 2021Article
- Quasi-essentiality of RNase Y in Bacillus subtilis is caused by its critical role in the control of mRNA homeostasis.Nucleic acids research · 2021Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Ribonuclease P (RNase P) is essential for the 5'-end maturation of tRNAs in all kingdoms of life. In Escherichia coli, temperature sensitive mutations in either its protein (rnpA49) and or RNA (rnpB709) subunits lead to inviability at nonpermissive temperatures. Using the rnpA49 temperature sensitive allele, which encodes a partially defective RNase P at the permissive temperature, we show here for the first time that the processing of RNase P-dependent polycistronic tRNA operons to release pre-tRNAs is the essential function of the enzyme, since the majority of 5'-immature tRNAs can be aminoacylated unless their 5'-extensions ≥8 nt. Surprisingly, the failure of 5'-end maturation elicits increased polyadenylation of some pre-tRNAs by poly(A) polymerase I (PAP I), which exacerbates inviability. The absence of PAP I led to improved aminoacylation of 5'-immature tRNAs. Our data suggest a more dynamic role for PAP I in maintaining functional tRNA levels in the cell.
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